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If you purchase all the components you need to finish one Western blot separately, for the average market price, the total cost for one blot will be approximate $24. GenScript’s complete ONE-HOUR Western kits contain all the reagents ,membrane, HRP substrate and X-Ray film.
Cost comparison between GenScript’s ONE-hour ONE-HOUR WesternTM and traditional Western (for one blot).
| Kit Components |
Traditional Western |
GenScript ONE-HOUR Western |
Membrane (1 sheet) |
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All in One Package |
| Substrate (6 ml) |
$6.62 |
Blocking Solution (20 ml) |
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| Washing Buffer (125 ml) |
$1.79 |
|
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| Secondary Antibody |
$1.5 |
Total Time per Test |
4.5 hours |
1 hour, Priceless |
| Total Cost per Test |
$24.13 |
$19.50 |
What's new for the 2nd generation ONE-HOUR WesternTM technology?
1. GenScript's second generation ONE-HOUR WesternTM technology revolutionizes the protein detection process.
By incorporating a new proprietary blocking reagent and a new supersensitive HRP substrate in the ONE-HOUR WesternTM Kits, we are proud to offer customers high quality results and higher sensitivity, which allow using much less antibodies. It works by effectively blocking non-specific binding of primary and secondary antibodies and by selectively enhancing binding between primary and secondary antibodies.
2. The 2nd generation ONE-HOUR WesternTM technology provides better sensitivity and lower background than ever!
3. The second generation ONE-HOUR WesternTM procedure is as simple as a-b-c:
a: Pretreat your transferred blot with the mixture of Solution A and B for 5 min;
b: Incubate your blot with the mixture of your primary antibodies , solution WB-1 and WB-2 for 40 min (Pre-mixture primary antibody and WB-1 solution 40min, then add into WB-2 solution);
c: Develop the signal with the choice of HRP substrate or TMB substrate.
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