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If you purchase all the components you need to finish one Western blot separately, for the average market price, the total cost for one blot will be approximate $24. GenScript’s complete One-Step Western kits contain all the reagents ,membrane, HRP substrate and X-Ray film. The price per blot will be $19 after discount, not counting the significant time saved on running the actual procedure, searching around for reagents and optimizing the conditions.
Cost comparison between GenScript’s ONE-hour One-Step WesternTM and traditional Western (for one blot).
| Kit Components |
Traditional Western |
GenScript One-Step Western |
Membrane (1 sheet) |
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All in One Package |
| Substrate (6 ml) |
$6.62 |
Blocking Solution (20 ml) |
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| Washing Buffer (125 ml) |
$1.79 |
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| Secondary Antibody |
$1.5 |
Total Time per Test |
4.5 hours |
1 hour, Priceless |
| Total Cost per Test |
$24.13 |
$19.50 |
To introduce the 2nd generation One-Step WesternTM kits, we offer a 30% discount on selected kits, valid until 12/31/08.
What's new for the 2nd generation One-Step WesternTM technology?
1. GenScript's second generation One-Step WesternTM technology revolutionizes the protein detection process.
By incorporating a new proprietary blocking reagent and a new supersensitive HRP substrate in the One-Step WesternTM Kits, we are proud to offer customers high quality results and higher sensitivity, which allow using much less antibodies. It works by effectively blocking non-specific binding of primary and secondary antibodies and by selectively enhancing binding between primary and secondary antibodies.
2. The 2nd generation One-Step WesternTM technology provides better sensitivity and lower background than ever!
Besides that, it reduces your budget from $24 per blot to $19 after discount, plus priceless time saving.
3. The second generation One-Step WesternTM procedure is as simple as a-b-c:
a: Pretreat your transferred blot with the mixture of Solution A and B for 5 min;
b: Incubate your blot with the mixture of your primary antibodies , solution WB-1 and WB-2 for 40 min (Pre-mixture primary antibody and WB-1 solution 40min, then add into WB-2 solution);
c: Develop the signal with the choice of HRP substrate or TMB substrate.
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