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CRISPR gene editing service
CRISPR genome editing service

CRISPR gene editing service

CRISPR is revolutionizing the field of gene editing. It has the potential to alter the course of research and drug discovery, by providing scientists with a powerful tool to change any gene, in any cell in a highly targeted manner and without introducing foreign DNA. The benefits of CRISPR/Cas9 over previous forms of gene editing, such as TALENs and zinc finger nuclease (ZFN), are that it is much simpler to implement and has higher efficiency at performing bi-allelic gene modifications.

GenScript is pleased to introduce GenCRISPR™, a full CRISPR based gene editing service, to produce a genetically modified cell using any mammalian cell line and targeting any gene.  Our scientists are experts at performing gene editing with CRISPR, from designing gRNA constructs for CRISPR to transfection and single clone generation of a wide range of cells, including difficult to transfect and tumor cell lines.

Service details

GenCRISPR™ cell line service Cell lines Deliverables Timeline Starting price
EZ Knock-out cell line service
  • Transfection-based
  • Customers chose from list of easy-to-use cell lines Customers specify
  • Customers specify gene/locus region and cell line of interest*
A375, CHO-K1, HCT116, HEK293, HEK293T, Hela, HEP-2, U2OS
  • Single gene
  • Homogeneous biallelic knock-out cell line validated by sequencing. Validated gRNA-Cas9 constructs
  • Deliver a negative knock-out cell line as a control.
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability.
  • Biweekly update of project progress.
13-18 weeks
$6,000
Customized Knock-out cell line service
  • Viral or transfection based
  • Customer specifies gene/locus region and cell line of interest*
Any cell line of interest
  • Single or multiplex genes
  • Homogeneous biallelic knock-out cell line validated by sequencing
  • Validation of biallelic knock-out cell line at mRNA or protein level upon request
  • Validated gRNA-Cas9 constructs
  • Deliver a negative knock-out cell line as a control.
  • 3 cryovials of cells per each clone, mycoplasma-free and 90% of viability.
  • Biweekly update of project progress
16-20 weeks (easy-to-handle lines)

20-30 weeks (difficult-to-handle lines)
$8,000
Knock-out cell pool
  • Viral or transfection based
  • Customer specifies gene/locus region and cell line of interest*
Any cell line of interest
  • Single or multiplex genes
  • Stable cell pool containing knock-out clones validated by sequencing
  • Validation of knock-out cell pool at mRNA or protein level upon request
  • Validated gRNA-Cas9 constructs
  • 3 cryovials of cells, mycoplasma-free and 90% of viability
  • Biweekly update of project progress
9-15 weeks
$4,500

*It is preferred that customers provide their own cell lines, but cell lines can also be purchased by GenScript for an additional fee.
**Pricing includes cost for one cell line derived from a single clone with one target gene knocked out. An additional fee will be applied for additional clones.

Optional services

Optional Services Description
Additional single clones
An additional single clone, from the same pool as the first single clone, will be characterized and sent to the customer. Additional clone will be target sequence analyzed and included in the final report
Western blot
Validation of biallelic knock-out cell line or cell pool at protein level
RT-PCR
Validation of biallelic knock-out cell line at protein level by sequencing cDNA
Q-PCR 
Validation or characterization of knockout cell line or cell pool by measuring mRNA level change.
Off-target QC data
Top 10 of potential off-target genes will be provided to the customer, and customer will choose which genes need to be sequenced. A detailed report, with sequencing of off-target regions will be provided to the customer
Growth curves 
Characterization of knock-out cell line growth by monitoring cell growth rate
Functional validation
  • Our in-vitro assay platform provides these services: Growth and apoptosis
  • GPCR and ion channel assays
  • In-cell western
  • Tumor cell line profiling

CRISPR workflow

CRISPR gene editing workflow

Case studies

Using viral-based CRISPR to develop a homozygous K-ras knock-out in HCT116 cells See more »

Using CRISPR to develop a glutamine synthetase (GS) knockout DG44 cell line See more »

Tested cell lines

GenScript has extensive experience in custom cell line editing. The tested cell lines include:

Cell line Description
A375 Human malignant melanoma
A549 Human lung adenocarcinoma epithelial
CHO Hamster ovary epithelial
Colo320 Human colon adenocarcinoma
Expi293F  Bioproduction cell line
HACAT Human keratinocyte
HCT116 Human colon epithelial
HEK293 Human embryonic kidney
Hela Human cervix epithelial
HEP-2 Human laryngeal carcinoma, Hela contaminant
HepaRG Human hepatic stem cell
HepG2 Human liver epithelial
HK2 Human kidney proximal tubule 
HT-29 Human colon epithelial
Jurkat Human T Lymphocyte
MDCKII Canine kidney epithelial
NCI-H1299 Human lung lymph node
TF-1 Human erythroleukemic 
U2OS Human osteosarcoma
U937 Human lymphocyte 
Vero  Monkey kidney epithelial
Vero76 Monkey kidney epithelial
Wit49 Human Wilms tumor 
WSU-DLCL2 Human B cell lymphoma 
WT macrophage cell lines  

Related services

Quotations and Ordering

For quotation requests:

To place an order:

Legal Statement of GenCRISPR Services and Products (Updated on July 28, 2015):

  1. GenCRISPR™ services and products are covered under US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents and licensed from Broad Institute, Inc. Cambridge, Massachusetts.
  2. .The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude (a) any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use, (b) any human germline modification, including modifying the DNA of human embryos or human reproductive cells, (c) any in vivo veterinary or livestock use, or (d) the manufacture, distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of, or as, a testing service, therapeutic or diagnostic for humans or animals.
  3. The purchase of the GenCRISPR Services and Products coveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from GenCRISPR services and any related material solely for Research Purposes only, not for any Commercial Purposes.

Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you. However, you may also contact us by email , phone, or fax.

 
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