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CRISPR gene editing service

CRISPR Gene Editing Services

CRISPR genome editing service

CRISPR is revolutionizing the field of gene editing. It has the potential to alter the course of research and drug discovery, by providing scientists with a powerful tool to change any gene, in any cell in a highly targeted manner and without introducing foreign DNA. The benefits of CRISPR/Cas9 over previous forms of gene editing, such as TALENs and zinc finger nuclease (ZFN), are that it is much simpler to implement and has higher efficiency at performing bi-allelic gene modifications.

GenScript is pleased to introduce GenCRISPR™, a full CRISPR-based gene editing service, to produce a genetically modified cell using any mammalian cell line and targeting any gene.  Our scientists are experts at performing gene editing with CRISPR, from designing gRNA constructs for CRISPR to transfection and single clone generation of a wide range of cells, including difficult-to-transfect and tumor cell lines.

GenCRISPR™ cell line service Cell Lines Timeline Starting Price^ Deliverables and Details

EZ Knock-out cell line service
(SC1755)

  • Transfection-based
  • Customers choose a cell line from a list of 130 cell lines.
  • FACS sorting to enrich the cell pool to reduce the risk of plasmid integration.
  • Customers specifies gene/locus region and cell line of interest*
30 easy-to-handle cell lines 13-16 weeks $6,000
  • Single gene editing KO cell line
  • Two full-allelic knock-out cell lines validated by sequencing
  • Validated gRNA-Cas9 constructs
  • Negative knock-out cell line as a control
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability.
  • Biweekly update of project progress
100 medium difficulty cell lines 13-16 weeks $7,000

Customized Knock-out cell line service
(SC1652)

  • Transfection based
  • Customer specifies gene/locus region and cell line of interest^
  • Cas9 stable pool service for hard-to-transfect cell lines
Any cancer or immortalized cell line of interest

16-20 weeks
(easy-to-handle lines)

20-26 weeks
(difficult-to-handle lines)

$8,000
  • Single or multiplex genes editing KO cell line
  • *1-2 full-allelic knock-out cell line(s) validated by sequencing
  • Validation of full-allelic knock-out cell lines at mRNA or protein level upon request
  • Validated gRNA-Cas9 constructs
  • Negative knock-out cell line as a control
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability.
  • Biweekly update of project progress

Lenti-CRISPR KO Service
(SC1652-V)

  • Lentiviral based for difficult-to-transfect cell lines.
  • Customer specifies gene/locus region and cell line of interest*
  • Cas9 stable pool service
Any cancer or immortalized cell line of interest 16-26 Weeks $10,000
  • Single gene editing KO cell line
  • 1 full-allelic knock-out cell line(s) validated by sequencing
  • Cas9-stable cell pool as negative control
  • Validation of full-allelic knock-out cell lines at mRNA or protein level upon request
  • 2 cryovials of cells per each clone, mycoplasma-free and 90% viability.
  • Biweekly update of project progress

Knock-out cell pool
(SC1758)

  • Viral or transfection based
  • Customer specify gene/locus region and cell line of interest
Any cancer or immortalized cell line of interest 9-15 weeks $4,500
  • Single gene editing KO
  • Stable cell pool containing knock-out clones validated by sequencing
  • Validation of knock-out cell pool at mRNA or protein level upon request
  • Validated gRNA-Cas9 constructs
  • 2 cryovials of cells, mycoplasma-free and 90% viability.
  • Biweekly update of project progress.

*It is preferred that customers provide their own cell lines, but cell lines can also be purchased by GenScript for an additional fee.
^Prices may vary based on project specifications

CRISPR workflow

CRISPR gene editing workflow

Optional Services

Add-On Service Description
Reverse transcription (RT)-PCR Validate the knockout clones to carry the INDELs on CDS at mRNA level by sequencing the RT-PCR product. 
Western blot Validate the knockout clones by western blot. A validated antibody in the host cells with specific binding to target protein (RNAi or knockout) must be provided.
FACS analysis Validate the knockout clones by FACS analysis. A validated antibody in the host cells with specific binding to target protein (RNAi or knockout) must be provided. 
Promoter activity survey Survey the promoter activity (Cbh/CMV/EFS) in the host cells to maximize gRNA-Cas9 cleavage efficiency. 
Transfection optimization Survey the transfection methods for the hard-to-transfect cells to maximize gRNA-Cas9 cleavage efficiency.
Off-target analysis Characterize one knockout clone by sequencing the top 10 of potential off-target sites. 
One additional clone Sequence additional single clones to identify one additional knockout clone. QC and deliver one additional knockout clone.

Related services

Legal Statement of GenCRISPR Services and Products (Updated on July 28, 2015):

  1. GenCRISPR™ services and products are covered under US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents and licensed from Broad Institute, Inc. Cambridge, Massachusetts.
  2. The products and the reagents generated from these services shall be used as tools for research purposes, and shall exclude (a) any human or clinical use, including, without limitation, any administration into humans or any diagnostic or prognostic use, (b) any human germline modification, including modifying the DNA of human embryos or human reproductive cells, (c) any in vivo veterinary or livestock use, or (d) the manufacture, distribution, importation, exportation, transportation, sale, offer for sale, marketing, promotion or other exploitation or use of, or as, a testing service, therapeutic or diagnostic for humans or animals.
  3. The purchase of the GenCRISPR Services and Products coveys to the purchaser the limited, non-transferable right to use the products purchased and the reagents generated from GenCRISPR services and any related material solely for Research Purposes only, not for any Commercial Purposes.

Quotations and Ordering

CRISPR genome editing service

For quotation requests:

To place an order:

Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you. However, you may also contact us by email, phone, or fax.

 
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