Complete Affinity-Purified Peptide pAb Package (Rabbit) (Cat.No.: SC1031)

Affinity purification can isolate antibodies specific to the target peptide, and GenScript's process makes full use of this method. Peptides identical to those used for immunization are conjugated to agarose beads, which are subsequently exposed to sera for antibody binding. Nonspecific proteins are washed away. The specific anti-peptide antibodies bind to the beads and can then be eluted and collected. The following outlines GenScript antibody production and affinity purification process.

The use of affinity-purified antibodies gives significant advantages over raw sera and IgG fraction. Affinity-purified antibodies generate the clean signals vital to immunological applications such as immunostaining or immunocytochemistry.

GenScript's comprehensive polyclonal antibody production services are based on our advanced peptide synthesis and affinity-purification technology. GenScript's polyclonal services start at only $250. Our advanced peptide synthesis technology guarantees polyclonal antibodies with a final titer of 1:20,000 or better.

Traditional antibody production is based primarily on expressed or native proteins purified via gel electrophoresis or chromatography. Very few antibody generation systems employ the very best immunogen peptides, peptides that are synthesized de novo with thorough consideration of the specific needs of each specific antibody generation project. This may be due to the high prices of peptide synthesis and immunogen peptide sequence information. However, with the development of GenScript's efficient and precise peptide synthesis technology and bioinformatics, the costs of peptide production drop, putting peptide antigens within the reach of any lab.

GenScript synthetic peptides, owing in part to their minimal cost and high purity, can be used to produce antibodies in a variety of species, such as rabbits, goats, sheep, mice, rats, and chickens. In addition, during the synthesis process, post-translational modifications (PTMs) can be added at specific sites of peptide chains, increasing selectivity. Specifically, phosphorylation can be performed reliably, enabling the production of phosphorylation-state-specific antibodies, also known as phospho-specific antibodies.

In addition to lower prices, the use of synthetic peptides in the polyclonal antibody manufacturing process also has numerous advantages relative to the use of protein immunogens. Peptide immunogens give more freedom and flexibility, enabling the production of epitope-specific and epitope-limited antibodies by targeting specific epitopes and constructing specific assays to quantify the amount of the protein present.