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Home » Products » Immunology Products » Western Technology » One-Step Western Kits » Epitope Tag Detection Kit

One-Step WesternTM Epitope Tag Kit

Based on its breakthrough One-Step technology, GenScript provides One-Step WesternTM Epitope-Tag Kits, designed for fast and sensitive detection of epitope-tagged proteins by either Western or Dot blot analysis.

One-Step WesternTM Epitope-Tag Kits include DYKDDDDK-Tag Western Kit (L00215), GST-Tag Western Kit (L00211), HA-Tag Western Kit (L00214), His-Tag Western Kit (L00212), and Trx-Tag Western Kit (L00213). These kits contain all the reagents, membrane, HRP substrate necessary for a western or dot blot. Western blotting using these kits need neither primary antibody nor secondary antibody.

Western blot with one-step His-Tag Western kit

Key Features:

  • Easy to perform: This simple and rapid procedure yields a complete Western or Dot blot analysis in about one hour.
  • High sensitivity and low background: The kit is optimized to give high signal and low background.
  • Reproducible results: The kit produces highly reproducible results.
  • Pre-optimized: No optimization is needed.
  • Neither a primary antibody nor a secondary antibody is needed.

Applications:

The application of the One-Step WesternTM His-Tag Detection Kit are as follows:

  • Detections of His-tagged proteins
  • Confirmation of His-tagged protein expression
  • Screening of His-tagged protein expression for optimization

Example:

1. Western blot detection of Flag-tagged protein (expressed in E. coli cells)
Western blot with one-step flag-tag western kit Figure 2. Shown below is a western blot detection of DYKDDDDK-tagged protein using the GenScript One-Step WesternTM DYKDDDDK-Tag Kit (L00215). 100 ng, 50 ng, 25 ng, 13 ng, 6.3 ng, and 3.1 ng of Flag-BAP (Sigma, P7582) were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, and Lane 6, respectively. The blot was developed with the LumiSensor system included in the kit. The exposure time was two minutes.
2. Western blot detection of Trx protein
Western blot with One-Step Trx-Tag Western Kit Figure 3. Shown below is a western blot detection of recombinant Trx protein using the GenScript One-Step WesternTM Trx Detection Kit (L00213). 50 ng, 25 ng, 12 ng, 6.2 ng, 3.1 ng, 1.6 ng, and 0.8 ng of Trx were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 6, and Lane 7, respectively. The blot was developed with the LumiSensor system included in the kit. A few ng of Trx can be cleanly detected via western blot using the One-Step WesternTM Trx Detection Kit.
3. Western blot detection of GST protein
Western blot with One-Step GST-Tag Western Kit Figure 4. Shown below is a western blot detection of GST protein using the GenScript One-Step WesternTM GST Blot Kit (L00211). 500 ng, 167 ng, 55.6 ng, 18.5 ng, 6.17 ng, 2.05 ng and 0.69 ng of GST were loaded in Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, and Lane 7, respectively. The blot was developed with the LumiSensor system included in the kit.


Cat. No.NameSizePriceQuantity
L00215 One-Step WesternTM DYKDDDDK-Tag Kit 1 kit (10 assays) $350.00
L00211 One-Step WesternTM GST Detection Kit 1 kit (10 assays) $350.00
L00214 One-Step WesternTM HA-Tag Kit 1 kit (10 assays) $350.00
L00212 One-Step WesternTM His-Tag Kit 1 kit (10 assays) $350.00
L00213 One-Step WesternTM Trx Detection Kit 1 kit (10 assays) $350.00