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Cloning Vectors – GenEZ™ ORF Clones

GenEZ™ ORF clones offer the highest flexibility in ORF clone customization. Choose from our standard mammalian expression-ready cloning vector or from over 150 popular commercial cloning vectors for custom cloning.

Our standard vector pcDNA3.1+/C-(K)-DYK is an expression/transfection ready cloning vector for mammalian systems

Standard expression cloning vector


GenScript's GenEZ™ ORF clones are seamlessly cloned into our standard mammalian expression cloning vector, pcDNA3.1+/C-(K)-DYK, using our CloneEZ™ seamless cloning technology. Driven by a CMV promoter, pcDNA3.1+/C-(K)-DYK is equipped with a C-terminal DYKDDDDK tag for easy protein detection and purification. GenEZ™ ORF clones are delivered expression and transfection ready, saving researchers valuable time in the lab. There is no additional charge for cloning into pcDNA3.1+/C-(K)-DYK.

See more detailed vector information & maps on available vectors in our vector selection guide.


Expression System
Promoter
Bacterial Selection
Copy number
Epitope tag
Length
Mammalian
CMV
Ampicillin
High
C-DYK
5444

Custom cloning vectors

For enhanced flexibility, GenEZ™ ORF clones can be directly custom cloned into one of over 150 expression vectors that we offer compatible with mammalian or bacterial systems including pcDNA, pET, pPIC, and pUC vectors series.

In addition, for GenEZ™ ORFs cloned into custom vectors can be further customized by the addition of up to 30 bp of 5' or 3' flanking sequence – free of charge.

Search Your ORF cDNA Clones

Search Help

Search Help

Your can enter the following as search terms:

  • Entrez Gene ID (e.g. 7157)
  • gene symbol (e.g. TP53)
  • gene name (e.g. tumor protein p53)
  • gene synonyms (e.g. FLJ92943)
  • Ensembl ID (e.g. ENSG0000141510)
  • RefSeq Accession (e.g. NM_000546)
  • Species can be input after the keyword, using format "keyword [species:$species]" where $species can be name of species (like human or rat) or taxon id (like 9606).

Get more search, click here.

* Ordering your ORF clone starts with searching for the wild type gene sequence in our ORF clone database


ORF cloning vectors maps*

(Click any vector name to download the vector map in PDF)

Mammalian
pcDNA3.1(+)
pcDNA3.1(-)
pcDNA3.1(+)_myc-His A
pcDNA3.1(+)_myc-His B
pcDNA3.1(+)_myc-His C
pcDNA3.1(-)_myc-His A
pcDNA3.1(-)_myc-His B
pcDNA3.1(-)_myc-His C
pCI-Neo
pcDNA3.1+C-DYK
pcDNA3.1+C-HA
pcDNA3.1+C-6His
pcDNA3.1+C-Myc
pcDNA3.1+N-DYK
pcDNA3.1+N-HA
pcDNA3.1+N-6His
pcDNA3.1+N-Myc
pcDNA3.1+N-GST(Thrombin)
pcDNA3.1+N-GST(TEV)
pcDNA3.1/Hygro(+)
pcDNA3.1/Hygro(-)
pcDNA3.1/Zeo (+)
pcDNA3.1/Zeo (-)
pCMV-3Tag-1a
pCMV-3Tag-2a
pCMV-3Tag-3a
pCMV-3Tag-4a
pcDNA3.1+C-eGFP
pcDNA3.1+N-eGFP
pcDNA3.1+P2A-eGFP
pcDNA3.1+P2A
pcDNA3.1+N-DYK-P2A
pcDNA3.1+C-DYK-P2A
pCMV-3Tag-1a-P2A
pCMV-3Tag-3a-P2A
  Bacterial
pBluescript II KS(-)
pBluescript II KS(+)
pBluescript II SK(-)
pBluescript II SK(+)
pET-3a
pET-3b
pET-3c
pET-3d
pET-9a
pET-11a
pET-11b
pET-11c
pET-11d
pET-14b
pET-15b
pET-16b
pET-17b
pET-19b
pET-20b(+)
pET-21a(+)
pET-21b(+)
pET-21d(+)
pET-22b(+)
pET-23a(+)
pET-24a(+)
pET-24b(+)
pET-24c(+)
pET-24d(+)
pET-25b(+)
pET-26b(+)
pET-27b(+)
pET-28a(+)
pET-28b(+)
 
pET-28c(+)
pGEX-2TK
pGEX-4T-1
pGEX-4T-2
pGEX-4T-3
pGEX-5X-1
pGEX-5X-2
pGEX-5X-3
pGEX-6P-1
pGEX-6P-2
pGEX-6P-3
pMAL-c4x
pMAL-c5E
pMAL-c5x
pMAL-p5E
pMAl-p5g
pMAl-p5x
pQE-1
pQE-60
pGS-21a
pETDuet-1
pCDFDuet-1
pRSFDuet-1
pCOLADuet-1
pGEX-4T-1-H(RBS)
pGEX-4T-1-M(RBS)
pGEX-5X-1-H(RBS)
pGEX-5X-1-M(RBS)
pGEX-6P-1-H(RBS)
pGEX-6P-1-M(RBS)
pMAL-c4x-1-H(RBS)
pMAL-c4x-1-M(RBS)
pET-29a(+)
pET-29b(+)
pET-29c(+)
PET-30a(+)
PET-30b(+)
 
PET-30c(+)
PET-31b(+)
pET-32a(+)
pET-32b(+)
pET-41a(+)
pET-41b(+)
pET-41c(+)
pET-42a(+)
pET-42b(+)
pET-42c(+)
pET-43.1a(+)
pET-43.1b(+)
pET-45b(+)
pET-50b(+)
pET-51b(+)
pET-52b(+)

Yeast
pAO815
pPIC 3.5k
pPIC9
pPIC9K
pPICZA
pPICZB
pPICZC
pPICZalphaA
pPICZalphaB
pPICZalphaC
pESC-TRP
pESC-URA
pESC-HIS
pESC-LEU
Baculovirus
pBacPAK8
pBacPAK9
pAcG2T
pAcHLT A
pAcHLT B
pAcHLT C
pAcGHLT A
pAcGHLT B
pAcGHLT C
pAcSG2
pBAC-1
pFastBac1
pFastBacHT-A
pFastBacHT-B
pFastBacHT-C
pFastBac-Dual

*Not all vectors maps shown here. Vectors not shown may need to be shipped by the customer to GenScript for ORF custom cloning.

 
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