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Custom Guaranteed Antibody Service

Custom Guaranteed Antibody Service

GenScript has a unique antibody production technology. We intend to use this new service package: Western-GUARANTEED peptide polyclonal antibody package to satisfy your need for quality antibodies that generate positive Western blot results. Our confidence is backed up by our track record of having produced thousands of successful custom polyclonal antibodies with a success rate over 98%.

Our Western guarantee is possible because of proprietary OptimumAntigenTM design tool, nanotechnology, and proprietary T-MaxTM adjuvant. Full antibody production documentation including quality assurance comes with the delivery. GenScript's experienced and knowledgeable staff is there to help you with the antibody you need for your research.

SC1390: Western-GUARANTEED Peptide Polyclonal Antibody Package (Rabbit)

Package includes:
You will receive
Time Frame:
  • Peptide design and synthesis
      >85% purity, 15 mg
  • Peptide-KLH conjugation
  • 6 rabbits immunization, Pre-immune bleeds and test bleeds
  • Affinity purification and ELISA results
  • Western blot test
  • Lyophilization
  • 1-2 ml of pre-immune serum (lyophilized)*
  • 3-5 mg purified antibodies (lyophilized) from 2 best rabbits
  • 5 mg peptide
  • MS and HPLC reports
  • Western blot, ELISA titer reports
  • Total 16-18 weeks


* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided.

Shown below are Western blot results for endogenous protein detection


Western blot results
Marker: EasyWestern Protein Standard
Lane 1: Hela cell lysate
Lane 2: HEK293 cell lysate
Lane 3: Mouse brain cell lysate
Lane 4: Rat brain cell lysate
Primary antibody: 1 µg/ml Rabbit Anti-β-Tubulin III Polyclonal Antibody
Secondary antibody: IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
 

Western blot results
Marker: EasyWestern Protein Standard
Lane 1: Hela cell lysate
Lane 2: HEK293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: K562 cell lysate
Primary antibody: 1 µg/ml Rabbit Anti-Cofilin Polyclonal Antibody
Secondary antibody: IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

Find more details and service records

Quotation and Ordering:

  To design antigen and evaluate Western guarantee, please download & complete Western-GUARANTEED Information Sheet
  To request a quotation, please download and complete our Quick Quotation Request
   To order a pAb, please download & complete our Polyclonal Antibody Order Form

Please email or fax all quotation requests or completed order forms (including the Western-GUARANTEED Information Sheet) to GenScript.

  If you have any questions or would like to inquire about the status of your order, please contact us by phone, email, fax, or via our Secure Web Server.

Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.

Email: antibody@genscript.com
Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188
Fax: 1-732-210-0262 1-732-885-5878