Immunoprecipitation assays are fundamental for studying complex protein interactions that regulate cell physiology, gene expression and drive various types of disease. While agarose A/G beads have been the prevailing choice for quite some time, many labs have recently made the switch to Magnetic Beads after seeing results that are cleaner, more consistent and require less time. Just like agarose beads, magnetic beads bind to the Fc region of IgG as a means to isolate specific proteins and protein complexes from cell lysate. The advantage of magnetic beads is that they aggregate without the stress of centrifugation, which can significantly increase the yield of delicately attached protein complexes. Additionally, magnetic beads are easy to separate from supernatant by simply using a magnetic source close to your tube. This will allow the removal of unwanted supernatant without disturbing your protein pellet. Magnetic beads are also smaller and nonporous compared to agarose beads, so nonspecific binding and background signal is minimized.
Magnetic beads provide the same ability to isolate protein complexes as agarose beads with fewer steps and easier separation of pellet from the supernatant. Together, this translates to less experimental variability, reproducible data and confidence in your results. GenScript MagBeads are optimally designed for high capacity/Fc-specific IgG binding for immunoprecipiatation assays and small scale protein purification. Choose from our Protein A, Protein G, Protein A/G, or specially tagged magnetic beads and experience cleaner results in a shorter amount of time.
- Quick,easy and convenient magnetic separation procedures
- Extremely gentle sample handling with no loss of your target
- High binding capacity and reproducible results
- Ideal for immunoprecipitation and micro-scale protein and antibody purification
- Immunoprecipitation and micro-scale antibody purification
- Micro-scale purification of His and GST tagged protein
- Fast and flexible isolation of antibody and target protein
- Product Details
- Selection Guide
- Customer Citations
|Application ||Product ||Technology ||Binding capacity ||Size ||Cat.No. |
|Immunoprecipitation and micro-scale antibody purification ||Protein A MagBeads ||Protein/peptide capture through primary antibody ||>10 mg rabbit IgG/ml settled beads ||4 ml ||L00273 |
|Protein G MagBeads ||> 10 mg goat IgG/ml settled beads ||2 ml ||L00274 |
|Protein A/G MagBeads ||> 10mg goat or rabbit IgG/ml settled beads ||2 ml ||L00277 |
|Micro-scale purification of His-tagged proteins ||Ni-charged MagBeads ||His-tagged protein capture through pre-charged nickel ion ||5 - 20 mg 6×His-tagged protein (27kD)/ml settled beads ||8 ml ||L00295 |
|Micro-scale purification of GST fusion proteins ||Glutathione MagBeads ||GST fusion protein capture through reduced glutathione ||20 - 30 mg GST/ml settled beads ||8 ml ||L00327 |
|Immunoprecipitation and purification of biotinylated molecules ||Streptavidin MagBeads ||Biotinylated molecule capture through pre-coupled streptavidin ||> 60 nmol free biotin /ml settled beads ||2 ml ||L00424 |
|* Each size includes 75% Storage Buffer. |
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