Custom Recombinant Protein Services
Advance your proteomic workflow and remove bottlenecks in protein expression and purification! GenScript custom recombinant protein service is committed to solve your problems and speed your projects. After years of experience in protein services, we bring to the table unique protein chemistry expertise, large-scale fermentation capacity and unparalleled BacPower TM
guaranteed package. As your 24-hour off-campus protein core facility,
we offer multi-system expression for all kinds of proteins, chemical
protein synthesis and a full range of purification options.
- Services
- Competitive Advantages
- Successful Examples
- Customer Publications
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1.Most popular: Guaranteed Protein Expression Package ---3 mg purified soluble protein from $2200 (Cat.No. SC1253)
Package includes: |
Deliverables: |
Timeline: |
- Subclone
- Expression optimization and verification
- Expression and purification
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- Expression construct(s)
- 3 mg or more of purified soluble tagged protein
- QC data
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3.5~5 weeks |
2. Bacterial Expression System (Cat.No. SC1256)
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Specifications:
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Deliverables: |
Timeline: |
- Subcloning; Expression evaluation and optimization
- SDS-PAGE and Western-blot analysis
- One-step affinity or multiple steps of purification, protein purity available from 75% to >95%
- Tag removal and Endotoxin removal
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- Expression construct(s)
- 3 mg or more of purified soluble protein
- QC data
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4~6 weeks |
3. Yeast Expression System (Cat.No. SC1257)
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Specifications:
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Deliverables: |
Timeline: |
- Subcloning; Generation of recombinant yeast vectors
- Expression evaluation and optimization in various host strains
- One-step affinity or multiple steps of purification
- SDS-PAGE and Western-blot analysis
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- Cell pellet or purified soluble protein
- QC data
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6~9 weeks |
4. Baculovirus Expression System (Cat.No. SC1258)
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Specifications:
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Deliverables: |
Timeline: |
- Generation of recombinant bacmid DNA and high-titer viral stock
- Determination and confirmation of virus titer using qPCR or plaque assay
- Infection of insect cells (Sf9,Sf21,Hi5) with high-titer virus stocks
- One-step affinity or multiple steps of purification
- SDS-PAGE and Western-blot analysis
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- High-titer viral stock
- Cell pellet / media or purified soluble protein
- QC data
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6~10 weeks |
5. Mammalian Expression System (Cat.No. SC1259/SC1260)
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Capacity:
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Deliverables: |
Timeline: |
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- Expression Vectors
- Fusion protein or tag-free protein
- Stable protein expression cell lines
- QC data
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3~4 weeks /Assessed by project |
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1. BacPowerTM technology and FoldArtTM Protein Refolding Technology
Hard-to-express and hard-to-dissolve proteins are specifically tackled by our proprietary BacPowerTM technology and FoldArtTM Protein Refolding Technology.
2. Extensive recombinant protein production experience:
We have delivered hundreds of difficult proteins including trans-membrane proteins, secreted proteins, proteases, and other enzymes. The recombinant proteins we produced have been successfully used in target validation, enzyme characterization, antibody production, high-throughput screening (HTS), structural study, and SAR (Structure-activity relationships).
3. Comprehensive services:
GenScript can take your project directly from gene synthesis, to protein expression, stable cell line generation, antibody production and assay development.
4. High quality services:
GenScript provides high quality protein expression services to meet the requirements of high purity, high activity and speedy delivery. Our deliverables are available at the purity of >95%, and the endotoxin level of lower than 1 EU/µg.
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1. Non-tagged protein purification
SDS-PAGE result shows that target protein with the size of 78 KDa has been obtained on lane 12, 13, 14.
2. Protein Refolding
3. Tag removal
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Partial list of publications cited the choice of our protein services
- Magali Moreau, Gyu In Lee, Yongzeng Wang, Brian R. Crane, and Daniel F. Klessig. AtNOS/A1 is a functional Arabidopsis thaliana cGTPase and not a nitric oxide synthase. J. Biol. Chem. Sep 2008
- Ashish Nagarsekar, Rachel S. Greenberg, Nirav G. Shah, Ishwar S. Singh, and Jeffrey D. Hasday. Febrile-Range Hyperthermia Accelerates Caspase-Dependent Apoptosis in Human Neutrophils. J. Immunol. Aug 2008; 181(4): 2636 - 2643
- Brando C, Ware LA, Freyberger H, Kathcart A, Barbosa A, Cayphas S, Demoitie MA, Mettens P, Heppner DG, Lanar DE.
Murine immune responses to liver-stage antigen 1 protein FMP011, a malaria vaccine candidate, delivered with adjuvant AS01B or AS02A.
Infect. Immun. Feb 2007; 75(2): 838-845
- Buchko GW, Ni S, Robinson H, Welsh EA, Pakrasi HB, Kennedy MA. Characterization of two potentially universal turn motifs that shape the repeated five-residues fold--crystal structure of a lumenal pentapeptide repeat protein from Cyanothece 51142. Protein Sci. Nov 2006; 15(11): 2579-2595
- Dieterich DC, Link AJ, Graumann J, Tirrell DA, Schuman EM.
Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT). Proc. Natl. Acad. Sci. USA. Jun 2006; 103(25): 9482-9487
See more publications following this link...
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Quotations and Ordering:
To request a quotation, please download and complete the Quotation/Order Form, and send it to us by email or fax.
To order, please download and complete the Quotation/Order Form and send it to us by email
or fax with a formal PO (Purchase Order) or credit card information.
You can also submit PO/credit card information by phone or via our secure online messaging system.
If submitting samples, please mail them together with a hard copy of the completed Quotation/Order Form to: Protein Services, GenScript, 120 Centennial Ave., Piscataway, NJ 08854, US.
For questions, or to inquire about the status of your order contact us by email, phone, fax or via our secure online messaging system.
Our customer service representatives are available 24 hours a day, Monday through Friday to assist you.
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Email: protein@genscript.com |
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Phone: 1-877-436-7274 (Toll-Free) 1-732-885-9188 |
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Fax: 1-732-210-0262 1-732-885-5878 |
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