Western Multiplex KitKit Description:Western blot analysis is one of the most common methods of detecting proteins and of determining specific protein concentrations in biological samples. However, loading errors and other human problems can confound this otherwise reliable system. An internal control is often needed to control and correct for such problems and to semi-quantitate protein expression levels. To date, the three most widely used internal controls are housekeeping proteins GAPDH, β-actin, and α-tubulin. However, to avoid crossover reactions, researchers usually need to perform two Western blot (by either cutting the membrane into two halves or by stripping the membrane after the first blot) to detect the target protein and the internal control separately. GeScript developed series of One-StepTM Western Multiplex Kits to provide a solution. Like other kits employing GenScript One-StepTM Western technology, it completes the blot in about one hour. The One-StepTM Western Multiplex Kits contain WestClearTM Nitrocellulose Membrane (0.2 μm) and LumiSensorTM Plus Chemiluminescent HRP substrate optimized for best results. WestClearTM Nitrocellulose Membrane and LumiSensorTM Plus Chemiluminescent HRP Substrate Kits are also available separately. Key features:
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