Western Multiplex Kit

Kit Description:

Western blot analysis is one of the most common methods of detecting proteins and of determining specific protein concentrations in biological samples. However, loading errors and other human problems can confound this otherwise reliable system. An internal control is often needed to control and correct for such problems and to semi-quantitate protein expression levels. To date, the three most widely used internal controls are housekeeping proteins GAPDH, β-actin, and α-tubulin. However, to avoid crossover reactions, researchers usually need to perform two Western blot (by either cutting the membrane into two halves or by stripping the membrane after the first blot) to detect the target protein and the internal control separately. GeScript developed series of One-Step^TM Western Multiplex Kits to provide a solution. Like other kits employing GenScript One-Step^TM Western technology, it completes the blot in about one hour.

The One-Step^TM Western Multiplex Kits contain WestClear^TM Nitrocellulose Membrane (0.2 μm) and LumiSensor^TM Plus Chemiluminescent HRP substrate optimized for best results. WestClear^TM Nitrocellulose Membrane and LumiSensor^TM Plus Chemiluminescent HRP Substrate Kits are also available separately.

Key features:

• Easy to perform: The One-Step^TM Western Multiplex Kit has fewer and simpler steps than other Western kits, leaving fewer chances for human error.
• Low background: The One-Step^TM Western Multiplex Kit contains WestClearTM Nitrocellulose Membrane and LumiSensor^TM Plus Chemiluminescent HRP Substrate Kit, optimized for low background.
• High sensitivity: The One-Step^TM Western Multiplex Kit is sensitivity is comparable to or better than that of the classical
  4.5-hour procedure, depending on the quality and amount of antibodies used.
• Reproducible results: The One-Step^TM Western Multiplex Kit produces highly reproducible results.
• No secondary antibody is needed: No extra GAPDH antibody is needed.
• The One-Step^TM Western Multiplex Kit needs far less optimization than the classical three-step method.

Product List: