Western Multiplex Kit Kit Description: Western blot analysis is one of the most common methods of detecting proteins and of determining specific protein concentrations in biological samples. However, loading errors and other human problems can confound this otherwise reliable system. An internal control is often needed to control and correct for such problems and to semi-quantitate protein expression levels. To date, the three most widely used internal controls are housekeeping proteins GAPDH, β-actin, and α-tubulin. However, to avoid crossover reactions, researchers usually need to perform two Western blots (by either cutting the membrane into two halves or by stripping the membrane after the first blot and reprobing it) to detect the target protein and the internal control separately. GenScript developed series of ONE-HOUR WesternTM Multiplex Kits to provide a solution. Like other kits employing GenScript ONE-HOUR WesternTM technology, it completes the blot in about one hour. The ONE-HOUR WesternTM Multiplex Kits contain WestClearTM Nitrocellulose Membrane (0.2 μm) and LumiSensorTM Plus Chemiluminescent HRP substrate optimized for best results. WestClearTM Nitrocellulose Membrane and LumiSensorTM Plus Chemiluminescent HRP Substrate Kits are also available separately.
Key features: - Fast: The ONE-HOUR WesternTM Multiplex Kits save about 8 hours of your time compared to classical stripping and reprobing procedure.
- Easy to perform: The ONE-HOUR WesternTM Multiplex Kits have fewer and simpler steps than other Western kits, leaving fewer chances for human error.
- Low background: The ONE-HOUR WesternTM Multiplex Kit contains WestClearTM Nitrocellulose Membrane and LumiSensorTM Plus Chemiluminescent HRP Substrate, optimized for low background.
- High sensitivity: The sensitivity of the ONE-HOUR WesternTM Multiplex Kits is comparable to or better than that of the classical stripping/reprobing procedure, depending on the quality and amount of primary antibodies used.
- Housekeeping gene and secondary antibodies are included.
- Instant success: The ONE-HOUR WesternTM Multiplex Kits need far less optimization than the classical stripping/reprobing method.
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