Alanine Scanning Library
Alanine scanning libraries are designed to identify the specific amino acid residues responsible for the peptide's function, stability, and conformation. Alanine, the smallest chiral amino acid, is sequentially substituted for each non-alanine residue one at a time. Subsequently, corresponding change in epitope activity can be measured. Substitution of key amino acid residue(s) with alanine causes changes in epitope binding activity. This library enables quick determination of each individual amino acid's contribution to the peptide's functionality.
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| |Overlapping Peptide | || |Alanine Scanning
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| |Truncation Library | || |Positional Scanning
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| |Random Library | || |Scrambled Library
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- Richardson PL. The determination and use of optimized protease substrates in drug discovery and development. Curr. Pharm. Des. 2002; 8(28): 2559-81.
- Morrison KL, and Weiss GA. Combinatorial alanine-scanning. Curr. Opin. Chem. Biol. Jun 2001; 5(3): 302-7.
- Levine KB, Hamill S, Cloherty EK, and Carruthers A. Alanine scanning mutagenesis of the human erythrocyte glucose transporter putative ATP binding domain. Blood Cells Mol. Dis. Jan-Feb 2001; 27(1): 139-42.
- Sidhu SS, and Kossiakoff AA. Exploring and designing protein function with restricted diversity. Curr. Opin. Chem. Biol. Jun 2007; 11(3): 347-54.