 THE™ cMyc Tag Antibody (Mouse) |  | The best quality in the industry, THE™ cMyc tag antibody is one member of GenScript's THE™ Elite antibodies. The immunogen of THE™ cMyc tag antibody is a synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH. THE™ cMyc tag antibody is suitable for detecting the expression level of c-Myc or its fusion proteins where the c-Myc tag is terminal or internal. This antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more. Key Features: - High Specificity: No non-specific binding
- High Effect: 1:20 000 diluted from 1.0 mg/ml can get good signal in WB analysis
- High Sensitivity: Detection level as low as 5 ng
- Broad Reactivity: Recognization of N-terminal, internal and C-teminal c-Myc tagged protein
- Long-term Stability: Stability of lyophilized form for at least two years
- Rich Applications: ELISA, Western blotting, Immunoprecipitation, Immunofluorescence, etc.
Ordering Information:
Related Information:
| Examples: 1. Sensitivity Detection |
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Sensitivity analysis of THE™ c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.
A: 0.2 μg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 μg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 μg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)
The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG | | | 2. Reactivity Test | | | | | | | Western blot discovers working concentration comparison of THE™ c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing GenScript A00704 posses excellent performance in WB assay with surprising antibody dilution.
Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 μg/ml) in assay.
The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG. | | | 3. Immunoprecipitation Application | | | Lane 1-4: 0.5, 0.2, 0.1, and 0.05 μg Multiple Tag Cell Lysate (GenScript, M0100) as western blot control
Lane 5-7: 1, 0.5, and 0.25 μg
THE™ cMyc tag antibody (Mouse) (GenScript, A00704) with 20 μg Multiple Tag Cell Lysate (GenScript, M0100) for immunoprecipitation analysis performed by One-Step IP-Western Kit (Mouse)(GenScript, L00232) | | | 4. Immunofluorescence Staining | | |
Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using THE™ cMyc tag antibody (Cat. No. A00704). |
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