THE™ cMyc Tag Antibody (Mouse) |
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The best quality in the industry, THETM cMyc tag antibody is one member of GenSctipt's THETM Elite antibodies. The immunogen of THETM cMyc tag antibody is a synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH. THETM cMyc tag antibody is suitable for detecting the expression level of c-Myc or its fusion proteins where the c-Myc tag is terminal or internal. This antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.
Key Features:
- High Specificity: No non-specific binding
- High Effect: 1:20 000 diluted from 1.0 mg/ml can get good signal in WB analysis
- High Sensitivity: Detection level as low as 5 ng
- Broad Reactivity: Recognization of N-terminal, internal and C-teminal c-Myc tagged protein
- Long-term Stability: Stability of lyophilized form for at least two years
- Rich Applications: ELISA, Western blotting, Immunoprecipitation, Immunofluorescence, etc.
Ordering Information:
Related Information:
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Examples:
1. Sensitivity Detection |
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Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.
A: 0.2 µg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 µg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 µg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)
The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG
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2. Reactivity Test |
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Western blot discovers working concentration comparison of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing GenScript A00704 posses excellent performance in WB assay with surprising antibody dilution.
Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 µg/ml) in assay.
The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG. |
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3. Immunoprecipitation Application |
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Lane 1-4: 0.5, 0.2, 0.1, and 0.05 μg Multiple Tag Cell Lysate (GenScript, M0100) as western blot control
Lane 5-7: 1, 0.5, and 0.25 μg
THETM cMyc tag antibody (Mouse) (GenScript, A00704) with 20 μg Multiple Tag Cell Lysate (GenScript, M0100) for immunoprecipitation analysis performed by One-Step IP-Western Kit (Mouse)(GenScript, L00232) |
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4. Immunofluorescence Staining |
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Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using THETM cMyc tag antibody (Cat. No. A00704). |
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