THETM His tag antibody has superior affinity, able to recognize 6xHis-, 5xHis-, and 4xHis-tags. Even if the 6xHis-tag is only partially exposed, it will still be identified and bound by THETM His tag antibody. THETM His tag antibody, which is produced from mice ascites and purified by protein A affinity column, also recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. Furthermore, THETM His tag antibody recognizes His Tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. THETM His tag antibody can be widely used in Western blot analysis, Dot blot analysis, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Key Features:
High Specificity: No non-specific binding
Superior Sensitivity: The most sensitive His Tag mAb in the market
1. Western Blot of 6xHis-tag Fusion Protein using THETM His Tag Antiboy (A00186)
Figure 1: Comparison of detection of 6xHis-tag Fusion Protein using THETM His Antibody, mAb, Mouse (GenScript, A00186, 0.1 μg/ml) with Mouse Anti-His mAb (Competitor A, 0.1 μg/ml) by Western blot.
Figure 2: Western blot analysis of N-terminal and C-terminal 6xHis-tag Fusion Protein using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 μg/ml).
2. Dot Blot Detection of 5xHis-GST in Whole Cell Lysates using THETM His Tag Antibody (A00186)
Figure 3: Comparison of Detection of 5xHis-GST in Whole Cell Lysates using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 μg/ml) with Mouse Anti-His mAbs (Competitor Q#1 and Q#2, 1 μg/ml) by Dot blot.
3. Western Blot Detection of 4xHis-GST in Whole Cell Lysates using THETM His Tag Antibody (A00186)
Figure 4: Comparison of detection of 4xHis-GST in Whole Cell Lysates using THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 μg/ml) with Mouse Anti-His mAb (Competitor B, 1 μg/ml) by Western blot.
4. Western blot analysis of immunoprecipitates using THETM His Tag Antibody (A00186)
Figure 5: Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THETM His Antibody, mAb, Mouse (GenScript, A00186).
5. Lot-to-lot consietency using Western Blotting
Figure 6: Consistency analysis of THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 μg/ml) with His-tagged fusion protein by Western blot.
6. Immunofluorescent staining using THETM His Tag Antibody (A00186)
Figure 7: Immunocytochemistry/Immunofluorescence analysis of HEK293 cells (A) and HEK293 cells transfected with His tagged fusion gene (B) using THETM His Antibody, mAb, Mouse (GenScript, A00186).
7. Flow cytometric analysis using THETM His Tag Antibody (A00186)
Figure 8: Flow cytometric analysis of HEK293 cells (A) and HEK293 cells transfected with His tagged fusion gene (B) using THETM His Antibody, mAb, Mouse (GenScript, A00186).
