THE™ His tag antibody has superior affinity, able to recognize 6xHis-, 5xHis-, and 4xHis-tags. Even if the 6xHis-tag is only partially exposed, it will still be identified and bound by THE™ His tag antibody. THE™ His tag antibody, which is produced from mice ascites and purified by protein A affinity column, also recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. Furthermore, THE™ His tag antibody recognizes His Tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. THE™ His tag antibody can be widely used in Western blot analysis, Dot blot analysis, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Key Features:
High Specificity - No non-specific binding
Superior Sensitivity - The most sensitive His Tag mAb on the market
Improved Reactivity- All C-terminal, internal, and N-terminal His Tags can be identified
Enhanced Binding - Detects even exposed 6xHis-Tags, 5xHis-Tags, and 4xHis-Tags
Long-term Stability - Lot-to-lot consistency and stable lyophilized form for at least two years
Product Details
Examples
Ordering Information:
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Examples:
1. Western Blot of 6xHis-tag Fusion Protein using THE™ His Tag Antiboy (A00186)
Figure 1: Sensivitity comparison of 6xHis-tag Fusion Protein by Western blot using His Antibody, mAb, Mouse from GenScript (A00186, 0.1 μg/ml) and Qiagen (34698 , 0.1 μg/ml).
Figure 2: Western blot analysis of N-terminal and C-terminal 6xHis-tag Fusion Protein using THE™ His Antibody, mAb, Mouse (A00186, 1 μg/ml).
2. Dot Blot Detection of 5xHis-GST in Whole Cell Lysates using THE™ His Tag Antibody (A00186)
Figure 3: Sensitivity comparison of 5xHis-GST in Whole Cell Lysates by Dot blot using His Antibody, mAb, Mouse from GenScript(A: A00186, 1 μg/ml) and Qiagen (B: 34698, 1 μg/ml and C:34660, 1 μg/ml) .
3. Western Blot Detection of 4xHis-GST in Whole Cell Lysates using THE™ His Tag Antibody (A00186)
Figure 4: Sensitivity comparison of 4xHis-GST in Whole Cell Lysates by Western blot using His Antibody, mAb, Mouse from GenScript (A00186, 1 μg/ml) and Qiagen (34698 , 1 μg/ml) .
4. Western blot analysis of immunoprecipitates using THE™ His Tag Antibody (A00186)
Figure 5: Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THE™ His Antibody, mAb, Mouse (A00186).
5. Lot-to-lot consietency using Western Blotting
Figure 6: Consistency analysis of THE™ His Antibody, mAb, Mouse (GenScript, A00186, 1 μg/ml) with His-tagged fusion protein by Western blot.
6. Immunofluorescent staining using THE™ His Tag Antibody (A00186)
Figure 7: Immunocytochemistry/Immunofluorescence analysis of HEK293 cells (A) and HEK293 cells transfected with His tagged fusion gene (B) using THE™ His Antibody, mAb, Mouse (A00186).
7. Flow cytometric analysis using THE™ His Tag Antibody (A00186)
Figure 8: Flow cytometric analysis of HEK293 cells (A) and HEK293 cells transfected with His tagged fusion gene (B) using THE™ His Antibody, mAb, Mouse (A00186).
