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A00044-100
100 μg
$69
A00044-500
500 μg
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IL-8 Antibody, mAb, Mousecustom antibody production
IL-8 Antibody, mAb, Mouse
Full Name IL-8 Antibody, mAb, Mouse
Abbreviated name-1
 human IL-8; human Interleukin-8; Interleukin-8; Neutrophil activating protein-1
Documents
IL-8 Antibody, mAb, MouseDocument-MSDS: 995_20051003104713.PDF (PDF)
IL-8 Antibody, mAb, MouseDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
IL-8 Antibody, mAb, Mouse zoomIL-8 Antibody, mAb, Mouse
Reference
Kurdowska AK, et al. Interleukin-8 and anti-interleukin-8 autoantibodies in gingival crevicular fluid from patients with periodontitis. J. Periodontal. Res. Feb 2003; 38(1): 73-78.

Mian BM, et al. Fully human anti-interleukin 8 antibody inhibits tumor growth in orthotopic bladder cancer xenografts via down-regulation of matrix metalloproteases and nuclear factor-kappaB. Clin. Cancer Res. Aug 2003; 9(8): 3167-3175.

Description
 IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72-amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. Recombinant human IL-8 is an 8,400 Da protein containing 72 amino acids. This antibody reacts with human Interleukin-8 (IL-8) protein. The immunogen used to produce the antibody is recombinant human IL-8 produced in E. coli containing 72 amino acids and having a molecular mass of 8,452 Da.
Ig Subclass
 Mouse IgG1
Storage
 The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
 Human,Reactivity to other species is not tested yet.
Concentration
 1 mg/ml in PBS, pH 7.4 with 0.02% sodium azide
Immunogen
 Purified recombinant human IL-8
Purification Method
 Protein G affinity column
Applications
 Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1 µg/ml
Western blot: 1 µg/ml
Fusion Partner
 Spleen cells are fused with SP2/0-Ag14 mose myeloma cells.
Conjugation
 Unconjugated
Host Species
 Mouse
Antigen Species
 Human
Note
 This antibody is produced in BALB/c mice.
Technical Manual
 ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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