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ACAT1 Antibody, mAb, Mouse
Full Name
ACAT1 Antibody, mAb, Mouse ACAT1 Antibody, mAb, Mouse
Abbreviated name-1
Mouse Anti ACAT1 mAb;
Documents
Description
ACAT1 is a homotetrameric enzyme that catalyzes the formation of cholesterol esters from cholesterol and long-chain fatty acyl coenzyme A. The gene encoding human ACAT1 maps to chromosome 1 and is expressed as a protein of 50 kDa that localizes to the endoplasmic reticulum (ER) in macrophages and in several tissues, including those of the liver, the adrenal glands, and kidneys. ACAT1 is involved in cellular cholesterol homeostasis as well as in foam cell formation and the subsequent progression of atherosclerosis.
Ig Subclass
Mouse monoclonal (IgG1)
Storage
The antibody remains stable for one year from the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. The aliquots will remain stable for three months if stored at 4°C.
Specificity
Recognize specifically human ACAT1 and that of other species not tested
Formulation
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Immunogen
N-terminal 104 amino acids of human ACAT1 protein
Clone ID
A00082.01
Purification Method
Immunoaffinity purification
Applications
Western blot: 1:1,000
ELISA: 1:2,000-1:5,000
Conjugation
Unconjugated
Species Reactivity
Human
Host Species
Mouse
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Guo ZY, et al. The active site His-460 of human acyl-coenzyme A:cholesterol acyltransferase 1 resides in a hitherto undisclosed transmembrane domain. J. Biol. Chem. Nov 2005; 280 (45): 37814-37826.

Yang L, et al. Human acyl-coenzyme A:cholesterol acyltransferase 1 (acat1) sequences located in two different chromosomes (7 and 1) are required to produce a novel ACAT1 isoenzyme with additional sequence at the N terminus. J. Biol. Chem. Oct 2004; 279 (44): 46253-46262.

Lee RG, et al. Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates. J. Lipid Res. Dec 2000; 41 (12): 1991-2001.

Pamela J. McFie.,et al. Murine Diacylglycerol Acyltransferase-2 (DGAT2) Can Catalyze Triacylglycerol Synthesis and Promote Lipid Droplet Formation Independent of Its Localization to the Endoplasmic Reticulum.J Biol Chem.2011Aug;286(32):28235 - 28246

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