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GAPDH Antibody, mAb, Mouse  |
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| Full Name |
GAPDH Antibody, mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-GAPDH mAb; |
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Description |
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling. |
Ig Subclass |
Mouse Monoclonal (IgG1) |
Storage |
The product will remain stable for one year after the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will remain stable for three months if stored at 4°C. |
Specificity |
Mouse Anti GAPDH is known to react with GAPDH form humans, cows, pigs, mice, rats and other mammals. It also recognizes avian GAPDH. |
Concentration |
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid. |
Immunogen |
Purified pig GAPDH |
Purification Method |
Immunoaffinity purification |
Applications |
Western blot at dilutions of 1:1,000 and immunofluorescence experiments at 1:100 dilutions respectively. It is very useful as a loading control for quantitative Western blotting. |
Conjugation |
Unconjugated |
Species Reactivity |
Human, cow, pig, rat and other mammals, avian |
Host Species |
Mouse |
Antigen Species |
Pig |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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Related Products
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1 |
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2 |
Polyclonal
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services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
|
Document-MSDS: 1254_20051122221158.PDF (PDF)