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GAPDH Antibody, mAb, Mouse

*This product has been discontinued! *
Product Aliases Mouse Anti-GAPDH mAb;
Description Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, and ER to Golgi vesicle shuttling.
Immunogen Purified pig GAPDH
Host Species Mouse
Conjugation Unconjugated
Purification Method Immunoaffinity purification

Formulation 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid.
Clone ID A00084.01
Ig Subclass Mouse Monoclonal (IgG1)
Specificity Mouse Anti GAPDH is known to react with GAPDH form humans, cows, pigs, mice, rats and other mammals. It also recognizes avian GAPDH.
Storage The product will remain stable for one year after the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will remain stable for three months if stored at 4°C.
Note Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Western blot at dilutions of 1:1,000 and immunofluorescence experiments at 1:100 dilutions respectively. It is very useful as a loading control for quantitative Western blotting.
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

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Martins RP.,et al. Pyroptosis and adaptive immunity mechanisms are promptly engendered in mesenteric lymph-nodes during pig infections with Salmonella enterica serovar Typhimurium.Vet Res.2013Dec;44(1):120

You can visit "Citations Database" for more citations. He Y.,et al. Tlr Agonists Stimulate Nlrp3-Dependent Il-1Β Production Independently Of The Purinergic P2X7 Receptor In Dendritic Cells And In Vivo.J Immunol.2013Jan;190:334 - 339

You can visit "Citations Database" for more citations. Niu L.,et al. Berberine attenuates lipopolysaccharide-induced impairments of intestinal glutamine transport and glutaminase activity in rat.Fitoterapia.2011Apr;82(3):323-30

You can visit "Citations Database" for more citations. Liu J, et al. MiR-142-3p Attenuates the Migration of CD4+ T Cells through Regulating Actin Cytoskeleton via RAC1 and ROCK2 in Arteriosclerosis Obliterans. PLoS One. 2014April;9(4):

You can visit "Citations Database" for more citations.

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