Catalog Products » Other Products » New Products » GFAP Antibody, mAb, Mouse
Email to GenScript

*This product has been discontinued! *
GFAP Antibody, mAb, Mouse
Full Name
GFAP Antibody, mAb, Mouse GFAP Antibody, mAb, Mouse
Abbreviated name-1
Mouse Anti-GFAP mAb; Anti GFAP (monoclonal)
Documents
Description
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Ig Subclass
Mouse Monoclonal (IgG1)
Storage
Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Specificity
Mouse Anti GFAP is known to react with human, cow, pig, mouse, rat and all other mammalian and avian species tested to date.
Formulation
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Immunogen
Purified pig spinal core GFAP
Clone ID
A00085.01
Purification Method
Immunoaffinity purification
Applications
Western blot at dilutions of 1:1,000 and Immunofluorescence experiments at 1:100 dilutions respectively. It is very useful as a loading control for quantitative Western blotting.
Conjugation
Unconjugated
Species Reactivity
Human, cow, pig, mouse, rat and all other mammalian and avian species tested
Host Species
Mouse
Antigen Species
Pig
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Chekhonin VP, et al. Monoclonal anti-GFAP antibodies: extraction, characteristics, and immunoenzyme assay. Bull Exp. Biol. Med. Aug 2001; 132(2): 772-775.

Poletaev AB, et al. Serum anti-S100b, anti-GFAP and anti-NGF autoantibodies of IgG class in healthy persons and patients with mental and neurological disorders. Autoimmunity. 2000; 32(1): 33-38.

Mecocci P, et al. Serum anti-GFAP and anti-S100 autoantibodies in brain aging, Alzheimer's disease and vascular dementia. J. Neuroimmunol. Mar 1995; 57(1-2): 165-170.

Vacca V.,et al. Botulinum toxin A increases analgesic effects of morphine, counters development of morphine tolerance and modulates glia activation and μ opioid receptor expression in neuropathic mice.Brain Behav Immun.2013Feb;

Marinelli S.,et al. The Analgesic Effect on Neuropathic Pain of Retrogradely Transported botulinum Neurotoxin A Involves Schwann Cells and Astrocytes.PLoS One.2012Otc;7(10):e47977

Related Products
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.