| Full Name |
LPL Antibody, mAb, Mouse
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Abbreviated name-1 |
Mouse Anti LPL (monoclonal); Mouse Anti LPL |
| Documents |
 Document-MSDS: 1266_20051122223023.PDF (PDF) |
| Document-MSDS: NaN3 MSDS.pdf (pdf) |
| Figures |
zoom |
| Reference |
Yin B, et al. Lipoprotein lipase regulates Fc receptor-mediated phagocytosis by macrophages maintained in glucose-deficient medium. J. Clin. Invest. Aug 1997; 100(3): 649-657.
Ikeda Y, et al. A sandwich-enzyme immunoassay for the quantification of lipoprotein lipase and hepatic triglyceride lipase in human postheparin plasma using monoclonal antibodies to the corresponding enzymes. J. Lipid Res. Oct 1990; 31 (10): 1911-1924.
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Description |
Lipoprotein lipase (LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion. Recently, it has also been shown that LPL promote the development of atherosclerosis through facilitation of monocyte adhesion to endothelial cells, stimulation of tumor necrosis factor alpha (TNF) secretion and induction of vascular smooth muscle cell proliferation. |
Ig Subclass |
Mouse IgG1 |
Storage |
The antibody remains stable for one year from date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will remain stable at 4°C for three months. |
Specificity |
Recognize specifically LPL from human and bovine |
Concentration |
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid |
Immunogen |
Human LPL whole protein |
Purification Method |
Immunoaffinity purification |
Applications |
Western blot: 1:100
ELISA: 1:200-500 in lipid metabolic research |
Conjugation |
Unconjugated |
Species Reactivity |
Human and bovine |
Host Species |
Mouse |
Antigen Species |
Human |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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