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LPL Antibody, mAb, Mouse
Full Name
LPL Antibody, mAb, Mouse LPL Antibody, mAb, Mouse
Abbreviated name-1
Mouse Anti LPL (monoclonal); Mouse Anti LPL
Documents
Description
Lipoprotein lipase (LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion. Recently, it has also been shown that LPL promote the development of atherosclerosis through facilitation of monocyte adhesion to endothelial cells, stimulation of tumor necrosis factor alpha (TNF) secretion and induction of vascular smooth muscle cell proliferation.
Ig Subclass
Mouse IgG1
Storage
The antibody remains stable for one year from date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will remain stable at 4°C for three months.
Specificity
Recognize specifically LPL from human and bovine
Formulation
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Immunogen
Human LPL whole protein
Clone ID
A00090.01
Purification Method
Immunoaffinity purification
Applications
Western blot: 1:100
ELISA: 1:200-500 in lipid metabolic research
Conjugation
Unconjugated
Species Reactivity
Human and bovine
Host Species
Mouse
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Yin B, et al. Lipoprotein lipase regulates Fc receptor-mediated phagocytosis by macrophages maintained in glucose-deficient medium. J. Clin. Invest. Aug 1997; 100(3): 649-657.

Ikeda Y, et al. A sandwich-enzyme immunoassay for the quantification of lipoprotein lipase and hepatic triglyceride lipase in human postheparin plasma using monoclonal antibodies to the corresponding enzymes. J. Lipid Res. Oct 1990; 31 (10): 1911-1924.

Pyle DM.,et al. IgE cross-linking critically impairs human monocyte function by blocking phagocytosis.J Allergy Clin Immunol.2013Feb;131(2):491-500

Sun L.,et al. Coronavirus papain-like proteases negatively regulate antiviral innate immune response through disruption of STING-mediated signaling.PLoS One.2012Feb;7(2):e30802

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