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SCD1 Antibody, mAb, Mouse  |
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| Full Name |
SCD1 Antibody, mAb, Mouse
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Abbreviated name-1 |
Anti SCD1 (monoclonal); Anti SCD1 |
| Documents |
 Document-MSDS: 6898_20081019221721.PDF (PDF) |
| Figures |
zoom |
| Reference |
Zhang Y, et al. Liver X receptor agonist TO-901317 upregulates SCD1 expression in renal proximal straight tubule. Am. J. Physiol. Renal. Physiol. May 2006; 290(5): F1065-1073.
Gutierrez-Juarez R, et al. Critical role of stearoyl-CoA desaturase-1 (SCD1) in the onset of diet-induced hepatic insulin resistance. J. Clin. Invest. Jun 2006; 116(6): 1686-1695.
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Description |
Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme (mouse 355 aa, rat 358 aa; ≈37,000 Da) is constitutively expressed in adipose tissue and is induced in liver in response to fat-free, high-carbohydrate diet. The SCD-1 expression is higher in female than in male mice. The repression of RNA levels and enzyme activity of hepatic SCD-1 by leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD-1 may be beneficial for the treatment of obesity, hepatic steatosis and other metabolic disorders. |
Ig Subclass |
Mouse monoclonal IgG2B |
Storage |
The antibody remains stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. The aliquot will remain stable at 4°C for three months. |
Specificity |
Reacts with human and mouse proteins |
Concentration |
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid |
Immunogen |
Full-length recombinant protein |
Purification Method |
Immunoaffinity purification |
Applications |
Western blot, ELISA, IP, and IHC in obesity research. Starting concentration for WB and Elisa are 1:1000，and for IP and IHC are 1:100. Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme (mouse 355aa, rat 358aa; ~37 KDa) is constitutively expressed in adipose tissue and is induced in liver in response to fat free, high carbohydrate diet. The SCD-1 expression is higher in female than male mouse. The repression of RNA levels and enzyme activity of hepatic SCD-1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD-1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders. |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse |
Host Species |
Mouse |
Antigen Species |
Human |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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