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SCD1 Antibody, mAb, Mouse
Full Name
SCD1 Antibody, mAb, Mouse SCD1 Antibody, mAb, Mouse
Abbreviated name-1
Anti SCD1 (monoclonal); Anti SCD1
Documents
Description
Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme (mouse 355 aa, rat 358 aa; ≈37,000 Da) is constitutively expressed in adipose tissue and is induced in liver in response to fat-free, high-carbohydrate diet. The SCD-1 expression is higher in female than in male mice. The repression of RNA levels and enzyme activity of hepatic SCD-1 by leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD-1 may be beneficial for the treatment of obesity, hepatic steatosis and other metabolic disorders.
Ig Subclass
Mouse monoclonal IgG2B
Storage
The antibody remains stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. The aliquot will remain stable at 4°C for three months.
Specificity
Reacts with human and mouse proteins
Formulation
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Immunogen
Full-length recombinant protein
Clone ID
A00093.01
Purification Method
Immunoaffinity purification
Applications
Western blot, ELISA, IP, and IHC in obesity research. Starting concentration for WB and Elisa are 1:1000,and for IP and IHC are 1:100. Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme (mouse 355aa, rat 358aa; ~37 KDa) is constitutively expressed in adipose tissue and is induced in liver in response to fat free, high carbohydrate diet. The SCD-1 expression is higher in female than male mouse. The repression of RNA levels and enzyme activity of hepatic SCD-1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD-1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders.
Conjugation
Unconjugated
Species Reactivity
Human, mouse
Host Species
Mouse
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Zhang Y, et al. Liver X receptor agonist TO-901317 upregulates SCD1 expression in renal proximal straight tubule. Am. J. Physiol. Renal. Physiol. May 2006; 290(5): F1065-1073.

Gutierrez-Juarez R, et al. Critical role of stearoyl-CoA desaturase-1 (SCD1) in the onset of diet-induced hepatic insulin resistance. J. Clin. Invest. Jun 2006; 116(6): 1686-1695.

Pyle DM.,et al. IgE cross-linking critically impairs human monocyte function by blocking phagocytosis.J Allergy Clin Immunol.2013Feb;131(2):491-500

Sun L.,et al. Coronavirus papain-like proteases negatively regulate antiviral innate immune response through disruption of STING-mediated signaling.PLoS One.2012Feb;7(2):e30802

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