|Product Aliases||Mouse Anti-SRY mAb; Anti SRY (monoclonal)|
|Description||The Sex-Determining Region (SRY) is a gene that encodes a transcription factor that is a member of the high mobility group HMG-box family of DNA binding proteins. The gene is located on the short arm of the Y chromosome and has a gene map locus - Yp11.3. Common synonyms of the gene name are Testis Determining Factor (TDF) and Testis Determining Factor on Y (TDY). The main function of the SRY protein is to initiate male sex determination as a transcriptional activator, which regulates a genetic switch in male development. The SRY HMG box recognizes DNA by partial intercalation in the minor groove. The SRY protein then binds to the DNA and distorts its shape (SRY protein in action). In doing so, it regulates genes that control the development of the testes.
Transcriptional activator which regulates a genetic switch in male development. It is responsible for initiating male sex determination. SRY HMG box recognizes DNA by partial intercalation in the minor groove.
|Immunogen||SRY-GST fusion protein|
|Purification Method||Immunoaffinity purification|
|Formulation||1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid|
|Ig Subclass||Mouse monoclonal IgG|
|Specificity||Recognize mouse SRY protein, others not tested|
|Storage||Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stale at 4°C for 3 months.|
|Note||Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.|
|Western blot: 1:1,000
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)