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SRY Antibody, mAb, Mouse
Full Name
SRY Antibody, mAb, Mouse SRY Antibody, mAb, Mouse
Abbreviated name-1
Mouse Anti-SRY mAb; Anti SRY (monoclonal)
Documents
Description
The Sex-Determining Region (SRY) is a gene that encodes a transcription factor that is a member of the high mobility group HMG-box family of DNA binding proteins. The gene is located on the short arm of the Y chromosome and has a gene map locus - Yp11.3. Common synonyms of the gene name are Testis Determining Factor (TDF) and Testis Determining Factor on Y (TDY). The main function of the SRY protein is to initiate male sex determination as a transcriptional activator, which regulates a genetic switch in male development. The SRY HMG box recognizes DNA by partial intercalation in the minor groove. The SRY protein then binds to the DNA and distorts its shape (SRY protein in action). In doing so, it regulates genes that control the development of the testes.
Transcriptional activator which regulates a genetic switch in male development. It is responsible for initiating male sex determination. SRY HMG box recognizes DNA by partial intercalation in the minor groove.
Ig Subclass
Mouse monoclonal IgG
Storage
Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stale at 4°C for 3 months.
Specificity
Recognize mouse SRY protein, others not tested
Formulation
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Immunogen
SRY-GST fusion protein
Clone ID
A00094.01
Purification Method
Immunoaffinity purification
Applications
Western blot: 1:1,000
ELISA: 1:2,000-5,000
Conjugation
Unconjugated
Species Reactivity
Mouse
Host Species
Mouse
Antigen Species
Mouse
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Sim H, et al. Defective calmodulin-mediated nuclear transport of the sex-determining region of the Y chromosome (SRY) in XY sex reversal. Mol. Endocrinol. Jul 2005; 19(7): 1884-1892.

Hossain A, Saunders GF. The human sex-determining gene SRY is a direct target of WT1. J. Biol. Chem. May 2001; 276(20): 16817-16823.

Yu X, Weissman SM. Characterization of the promoter of human leukocyte-specific transcript 1. A small gene with a complex pattern of alternative transcripts. J. Biol. Chem. Nov 2000; 275(44): 34597-34608.

Pyle DM.,et al. IgE cross-linking critically impairs human monocyte function by blocking phagocytosis.J Allergy Clin Immunol.2013Feb;131(2):491-500

Sun L.,et al. Coronavirus papain-like proteases negatively regulate antiviral innate immune response through disruption of STING-mediated signaling.PLoS One.2012Feb;7(2):e30802

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