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Catalog Products » Antibodies » Epitope Tag Antibodies » GST-tag Antibody, pAb, Rabbit

GST-tag Antibody, pAb, Rabbit

GST-tag Antibody, PAb, Rabbit

Western blot analysis of GST fusion protein using 1 μg/ml Rabbit Anti-GST-tag Polyclonal Antibody (GenScript, A00097)
The result was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)

GST-tag Antibody, PAb, Rabbit

Western blot analysis of immunoprecipitates from GST fusion protein lysates. GST-tagged protein was immunoprecipitated with
Rabbit Anti-GST-Tag Polyclonal Antibody (GenScript, A00097) and specificity was confirmed by immunprecipitation with rabbit IgG.
Western blot was performed using Rabbit Anti- GST-Tag Polyclonal Antibody (GenScript, A00097).

Product Aliases Rabbit Anti-GST-tag pAb;
Description GenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
Immunogen Recombinant Glutathione-S-Transferase from Schistosoma japonicum
Host Species Rabbit
Conjugation Unconjugated
Purification Method Immunoaffinity chromatography

Formulation 0.5 mg/ml in PBS, pH 7.4 with 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.
Ig Subclass Rabbit IgG
Specificity This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

GST-tag Antibody, PAb, Rabbit
GST-tag Antibody, PAb, Rabbit

Western blot analysis of GST fusion protein using 1 μg/ml Rabbit Anti-GST-tag Polyclonal Antibody (GenScript, A00097)
The result was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)

GST-tag Antibody, PAb, Rabbit
GST-tag Antibody, PAb, Rabbit

Western blot analysis of immunoprecipitates from GST fusion protein lysates. GST-tagged protein was immunoprecipitated with
Rabbit Anti-GST-Tag Polyclonal Antibody (GenScript, A00097) and specificity was confirmed by immunprecipitation with rabbit IgG.
Western blot was performed using Rabbit Anti- GST-Tag Polyclonal Antibody (GenScript, A00097).


Cat. No.
Product Name
Size
Price
Quantity
A00130-100 GST-tag Antibody [HRP], pAb, Rabbit
100 ug
$199.00
A00130-40 GST-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
A00202-100 GST-tag Antibody [Biotin], pAb, Rabbit
100 ug
$199.00
A00202-40 GST-tag Antibody [Biotin], pAb, Rabbit
40 ug
$99.00
A00865-100 THETM GST Antibody, mAb, Mouse
100 ug
$175.00
A00865-40 THETM GST Antibody, mAb, Mouse
40 ug
$69.00
A00865-200 THETM GST Antibody, mAb, Mouse
200 ug
$311.00
A00866-40 THETM GST Antibody [HRP], mAb, Mouse
40 ug
$74.00
A00867-40 THETM GST Antibody [Biotin], mAb, Mouse
40 ug
$74.00
A00867-200 THETM GST Antibody [Biotin], mAb, Mouse
200 ug
$333.00
A01380-40 GST-tag Antibody [HRP], pAb, Goat
40 ug
$99.00
L00207 GST Fusion Protein Purification Kit
1 kit
$139.00
L00411 GST tag ELISA Detection Kit
1 kit (1x96 well)
$298.00
A00866-100 THETM GST Antibody [HRP], mAb, Mouse
100 ug
$180.00
A01379-100 GST-tag Antibody, pAb, Goat
100 ug
$199.00
 
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You can visit "Citations Database" for more citations. Scott Bidlingmaier.,et al. Comprehensive Analysis of Yeast Surface Displayed cDNA Library Selection Outputs by Exon Microarray to Identify Novel Protein-Ligand Interactions.Mol Cell Proteomics.2011Mar;10(3):M110005116

You can visit "Citations Database" for more citations. Muda K.,et al. Parkinson-related LRRK2 mutation R1441C/G/H impairs PKA phosphorylation of LRRK2 and disrupts its interaction with 14-3-3.Proc Natl Acad Sci U S A.2013Dec;111(1):E34-E43

You can visit "Citations Database" for more citations. Bryan W. Pyle.,et al. Enzymatic synthesis of valerena‐4, 7 (11)‐diene by a unique sesquiterpene synthase from the valerian plant (Valeriana officinalis).FEBS J.2012Sep;279(17):3136-46

You can visit "Citations Database" for more citations. Shakeel U.R. Mir.,et al. Elevated progesterone receptor membrane component 1/sigma‐2 receptor levels in lung tumors and plasma from lung cancer patients.Int J Cancer.2012Jul;131(2):E1-9

You can visit "Citations Database" for more citations.







For more documents, please visit "Technical Support".

Cat. No. A00097
Size
Price
100 ug $199.00
 
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