GenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.5 µg/ml Western blot: 0.1-1 µg/ml Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized
Western blot analysis of GST fusion protein using 1 μg/ml Rabbit Anti-GST-tag Polyclonal Antibody (GenScript, A00097) The result was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Western blot analysis of immunoprecipitates from GST fusion protein lysates. GST-tagged protein was immunoprecipitated with Rabbit Anti-GST-Tag Polyclonal Antibody (GenScript, A00097) and specificity was confirmed by immunprecipitation with rabbit IgG. Western blot was performed using Rabbit Anti- GST-Tag Polyclonal Antibody (GenScript, A00097).