Catalog Products » Antibodies » Epitope Tag Antibodies » GST-tag Antibody, pAb, Rabbit
Cat. No.
Name
Size   
Price
Selected
A00097-100
100 μg
$198
GST-tag Antibody, pAb, Rabbit
Full Name GST-tag Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-GST-tag pAb;
Documents
GST-tag Antibody, pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
GST-tag Antibody, pAb, RabbitDocument-MSDS: 4098_20080227223847.PDF (PDF)
Figures
GST-tag Antibody, pAb, Rabbit zoomGST-tag Antibody, pAb, Rabbit
GST-tag Antibody, pAb, Rabbit zoomGST-tag Antibody, pAb, Rabbit
Description
GenScript Rabbit Anti-GST-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant version of glutathione-S-transferase from Schistosoma japonicum. Glutathione-S-transferase is an epitope tag in generating fusion proteins. Antibodies specific to fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The glutathione-S-transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
Ig Subclass
Rabbit IgG
Storage
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
This antibody can be used to detect GST-fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.
Formulation
0.5 mg/ml in PBS, pH 7.4 with 0.02% sodium azide
Immunogen
Recombinant Glutathione-S-Transferase from Schistosoma japonicum
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Schistosoma japonicum
Host Species
Rabbit
Antigen Species
Schistosoma japonicum
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
  • 6794_20081016054057.PDF
  • 12J000528_A00097_COA.pdf
  • 13C000583_A00097_COA.pdf
  • Citation
    Morari EC, et al. Role of glutathione-S-transferase and codon 72 of P53 genotypes in epithelial ovarian cancer patients. J. Cancer Res. Clin. Oncol. Aug 2006; 132(8): 521-528.

    Pyle DM.,et al. IgE cross-linking critically impairs human monocyte function by blocking phagocytosis.J Allergy Clin Immunol.2013Feb;131(2):491-500

    Sun L.,et al. Coronavirus papain-like proteases negatively regulate antiviral innate immune response through disruption of STING-mediated signaling.PLoS One.2012Feb;7(2):e30802

    Scott Bidlingmaier.,et al. Comprehensive Analysis of Yeast Surface Displayed cDNA Library Selection Outputs by Exon Microarray to Identify Novel Protein-Ligand Interactions.Mol Cell Proteomics.2011Mar;10(3):M110005116

    Muda K.,et al. Parkinson-related LRRK2 mutation R1441C/G/H impairs PKA phosphorylation of LRRK2 and disrupts its interaction with 14-3-3.Proc Natl Acad Sci U S A.2013Dec;111(1):E34-E43

    Bryan W. Pyle.,et al. Enzymatic synthesis of valerena‐4, 7 (11)‐diene by a unique sesquiterpene synthase from the valerian plant (Valeriana officinalis).FEBS J.2012Sep;279(17):3136-46

    Shakeel U.R. Mir.,et al. Elevated progesterone receptor membrane component 1/sigma‐2 receptor levels in lung tumors and plasma from lung cancer patients.Int J Cancer.2012Jul;131(2):E1-9

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