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ABCA1 Antibody, mAb, Mouse


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A00121 ABCA1 Antibody, mAb, Mouse
100 μg
$214.00



Abbreviated name-1 Mouse Anti-ABCA1 mAb; Anti ABCA1
Description This membrane-associated protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extracellular and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangiers disease and familial high-density lipoprotein deficiency.
Host Species Mouse
Conjugation Unconjugated
Purification Method Immunoaffinity purification

Formulation 1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Clone ID A00121.01
Ig Subclass Mouse monoclonal IgG1
Specificity Recognize specifically ABCA1 from human, mouse, and chicken
Storage The antibody will remain stable for 1 year from the date of shipment if stored at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquots will remain for three months if stored at 4°C.
Note Western blot, ELISA, IP, and IHC in lipid metabolic research. Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.

Starting concentration for WB and ELISA are 1:1000, for IP and IHC are 1:100. This antibody has been tested with direct ELISA, where a 96-well plate was coated with recombinant ABCA1 C-terminal protein. The antibody was then added to the wells and a goat anti-mouse IgG HRP conjugate was used for colour development. For ABCA1 quantification, a pair of anti-ABCA1 antibodies should be used to formulate a Sandwich ELISA and with a presence of an ABCA1 protein Standard. Optimal dilution to be determined by the end user.
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

ABCA1 Antibody, MAb, Mouse
ABCA1 Antibody, MAb, Mouse

Immunoreactivity of ABCA1 in adipose and pancreas tissue for ABCA1 protein.
A. Strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows).
Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown).
B. The negative control for Panel A. Original magnification: 330; hematoxylin counterstain.

ABCA1 Antibody, MAb, Mouse
ABCA1 Antibody, MAb, Mouse

Western blot analysis of ABCA1 induction by cholesterol (+)
in human fibroblast cells from different patients (1, 2, 3, 4).
Simultaneous bloting with anti-actin antibody was used for protein loading control.

ABCA1 Antibody, MAb, Mouse

ABCA1 Antibody, MAb, Mouse

ABCA1 Antibody, MAb, Mouse

ABCA1 Antibody, MAb, Mouse

ABCA1 Antibody, MAb, Mouse


Frank PG, et al. Caveolin-1 and regulation of cellular cholesterol homeostasis. Am. J. Physiol. Heart Circ. Physiol. Aug 2006; 291(2): H677-686.

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