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Trx-tag Antibody, pAb, Goat

Product Aliases Thioredoxin Antibody;
Description Thioredoxin (Trx) is small, multi-functional protein with oxidoreductase activity. It is a 12 kD redox protein with disulphide oxidoreductase activity. It has been used as an epitope tag in expression vectors.
GenScript Trx-tag Antibody, pAb, Goat is developed in goat using purified recombinant Trx-tag fusion protein. It is suitable for detecting fusion proteins that contain a Trx tag.
Immunogen Purified recombinant Trx-tag fusion protein
Host Species Goat
Conjugation Unconjugated
Purification Method Immunoaffinity chromatography

Formulation 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.
Ig Subclass IgG
Specificity GenScript Trx-tag Antibody, pAb, Goat specifically reacts with fusion proteins containing Trx epitope tags.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Trx-tag Antibody, PAb, Goat
Trx-tag Antibody, PAb, Goat

Western blot analysis of Trx-tagged fusion protein using Trx-tag Antibody, pAb, Goat (GenScript, A00129, 0.1 µg/ml)
The signal was developed with Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb (GenScript, A00178, 1:15,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 35 KD
Observed Size: 35 KD

Cat. No.
Product Name
A00178 Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb
1 mg

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Cat. No. A00129
40 ug $99.00
100 ug $199.00