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Trx-tag Antibody, pAb, Goat  |
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| Full Name |
Trx-tag Antibody, pAb, Goat
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Abbreviated name-1 |
Thioredoxin Antibody; |
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Description |
Thioredoxin (Trx) is small, multi-functional protein with oxidoreductase activity. It is a 12 kD redox protein with disulphide oxidoreductase activity. It has been used as an epitope tag in expression vectors.
GenScript Trx-tag Antibody, pAb, Goat is developed in goat using purified recombinant Trx-tag fusion protein. It is suitable for detecting fusion proteins that contain a Trx tag. |
Ig Subclass |
IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Trx-tag Antibody, pAb, Goat specifically reacts with fusion proteins containing Trx epitope tags. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
Immunogen |
Purified recombinant Trx-tag fusion protein |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1 µg/ml
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
E. coli, human |
Host Species |
Goat |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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Related Products
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
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Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 4110_20080228004819.PDF (PDF)