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Goat Anti-Rabbit IgG Antibody (H&L), pAb  |
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| Full Name |
Goat Anti-Rabbit IgG Antibody (H&L), pAb
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Abbreviated name-1 |
Goat Anti-Rabbit IgG (H&L); Anti-Rabbit IgG (H&L) |
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Description |
GenScript Goat Anti-Rabbit IgG (H&L) is highly purified from goat antiserum by immunoaffinity chromatography. It reacts with rabbit IgG heavy and light chains. |
Ig Subclass |
Goat IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript Goat Anti-Rabbit IgG (H&L) specifically recognizes rabbit IgG. It has not yet been tested in other species.
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Concentration |
1 mg/ml in PBS, pH7.4, 0.01% thimerosal
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Immunogen |
Full-length rabbit IgG (purified) |
Purification Method |
Immunoaffinity chromatography |
Applications |
GenScript Goat Anti-Rabbit IgG (H&L) is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy ,immunohistochemistry , and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA coating: 1:100-1:500
ELISA primary: 1:10,000-1:30,000
Western blot: 1:1,000-1:10,000
Other applications: user-optimized |
Conjugation |
Unconjugated |
Species Reactivity |
Rabbit |
Host Species |
Goat |
Antigen Species |
Rabbit |
Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 7004_20081020042355.PDF (PDF)