NF-H Antibody, pAb, Chicken

*This product has been discontinued!*

Neurofilaments can be defined as the intermediate or 10 nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.This antibody was raised in chickens against purified bovine NF-H neurofilaments.

Cat. No.	A00136
Size	Buy in bulk

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Overview Properties Background

Overview

Specificity	NF-H from human, rat, mouse, cat, chicken
Host Species	Chicken
Immunogen	To raise this antibody bovine intermediate filaments were prepared from spinal cords by the glycerol polymerization method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE-cellulose and the NF-H containing fractions were concentrated and further purified by preparative gel electrophoresis on a Biorad Prepcell.
Species Reactivity	Human, rat, mouse, cat, chicken

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Properties

Concentration	1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide. Frozen liquid
Purification	Immunoaffinity purification

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Background

Target Background

Neurofilaments can be defined as the intermediate or 10 nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins, known as NF-L, NF-M and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
This antibody was raised in chickens against purified bovine NF-H neurofilaments.

Synonyms

Chicken Anti-NF-H pAb; Anti NF-H

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Citations

1. Pelkonen A, et al. A modular brain-on-a-chip for modelling epileptic seizures with functionally connected human neuronal networks. Biosensors & bioelectronics. (2020-11)