NF-L Antibody, pAb, Chicken

*This product has been discontinued!*

Neurofilaments can be defined as the intermediate or 10 nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, α-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant and are biochemically stable.GenScript Chicken Anti-NF-L Polyclonal Antibody was generated against bovine spinal cord intermediate filaments. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, chickens were boosted with recombinant mouse NF-L purified from bacteria.

Cat. No.	A00140
Size	Buy in bulk

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Overview Properties Background

Overview

Specificity	NF-L from human, rat, mouse, cat, chicken
Host Species	Chicken
Immunogen	To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al., and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria.
Species Reactivity	Human, rat, mouse, cat, chicken

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Properties

Concentration	1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Purification	Immunoaffinity purification

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Background

Target Background

Neurofilaments can be defined as the intermediate or 10 nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes the neurofilament triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, α-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are quite abundant and are biochemically stable.
GenScript Chicken Anti-NF-L Polyclonal Antibody was generated against bovine spinal cord intermediate filaments. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, chickens were boosted with recombinant mouse NF-L purified from bacteria.

Synonyms

Chicken Anti NF-L pAb; Anti NF-L

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