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Vimentin Antibody, pAb, Chicken
Full Name
Vimentin Antibody, pAb, Chicken Vimentin Antibody, pAb, Chicken
Abbreviated name-1
Chicken Anti Vimentin pAb; Anti Vimentin
Documents
Description
Vimentin is protein subunit of the 10 nm or intermediate filaments protein found in many kinds of mesenchymal and epithelia cells, in many kinds of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system. Vimentin frequently forms copolymers with other intermediate filament proteins, such as GFAP (in many kinds of astrocytes), with desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. This antibody was raised in chickens against purified recombinant vimentin.
Ig Subclass
Chicken IgY
Storage
Stable for one year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4ºC for 3 months.
Specificity
Vimentin from human, rat, mouse
Formulation
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Immunogen
Recombinant vimentin purified from E. coli
Purification Method
Immunoaffinity purification
Applications
Immunofluorescence microscopy: 1:1,000
Conjugation
Unconjugated
Species Reactivity
Human, rat, mouse
Host Species
Chicken
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Ermakova S, et al. The intermediate filament protein vimentin is a new target for epigallocatechin gallate. J. Biol. Chem. Apr 2005; 280(17): 16882-16890.

Ishizaki M, et al. Expression of collagen I, smooth muscle alpha-actin, and vimentin during the healing of alkali-burned and lacerated corneas. Invest. Ophthalmol. Vis. Sci. Nov 1993; 34(12): 3320-3328.

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