Email to GenScript

*This product has been discontinued! *
MARCKS Antibody, pAb, Rabbit
Full Name
MARCKS Antibody, pAb, Rabbit MARCKS Antibody, pAb, Rabbit
Abbreviated name-1
Rabbit Anti-MARCKS pAb; Anti MARCKS
Documents
Description
Myristoylated alanine-rich C kinase substrate (MARCKS) translocates from the cytosol to the plasma membrane while mononucleated myoblasts fuse to form multinucleated myotubes. Rabbit anti MARCKS is an affinity purified rabbit polyclonal and used for neuroscience and cancer research. MARCKS is a member of the calmodulin binding protein family, is localised to the plasma membrane, forming cross-links with actin filaments. Phosphorylation by protein kinase C at key serine residues results in inhibition of binding to actin, phosphatidyl serine and calmodulin. MARCKS is thought to be involved in mitogenesis, phagocytosis and membrane trafficking.
Ig Subclass
Rabbit IgG
Storage
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Specificity
Myristoylated alanine rich C kinase substrate
Formulation
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Immunogen
Full length recombinant MARCKS (Myristoylated alanine rich C Kinase substrate) expressed in and purified from E. coli.
Purification Method
Immunoaffinity purified
Applications
Immunofluorescence: 1:250-1:500
ABC or other enzyme linked immunocytochemical procedures: 1:500
Western blot: 1:1,000
Conjugation
Unconjugated
Species Reactivity
Rat
Host Species
Rabbit
Antigen Species
Rat
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Kim SS, et al. Involvement of protein phosphatase-1-mediated MARCKS translocation in myogenic differentiation of embryonic muscle cells. J. Cell. Sci. Jun 2002; 115(Pt 12): 2465-2473.

Gallant C, et al. MARCKS is a major PKC-dependent regulator of calmodulin targeting in smooth muscle. J. Cell. Sci. Aug 2005; 118(Pt 16): 3595-3605.

Related Products
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.