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MAP2 Antibody, pAb, Chicken
Full Name
MAP2 Antibody, pAb, Chicken MAP2 Antibody, pAb, Chicken
Abbreviated name-1
Chicken Anti MAP2; Anti MAP2
Documents
Description
Microtubules are 25 nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP2 is made up of two ≈280,000 Da apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ≈70,000 Da on SDS-PAGE gels. Antibodies to MAP2 are excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons. Dendrite specific MAP found specifically in dendritic branching of neuron. This antibody does not bind to protein A or protein G.
Ig Subclass
Chicken IgY
Storage
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Specificity
MAP2 (Microtubule Associated Protein 2) from human, rat, mouse and bovine
Formulation
1 mg/ml in PBS, pH 7.4 with 0.05% sodium azide, frozen liquid
Immunogen
Recombinant MAP2
Purification Method
Immunoaffinity purification
Applications
Immunofluorescence: 1:5,000
Western blot using chemiluminescence: 1:50,000 or lower
Conjugation
Unconjugated
Species Reactivity
Human, rat, mouse and bovine
Host Species
Chicken
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Hirokawa N. 270K microtubule-associated protein cross-reacting with anti-MAP2 IgG in the crayfish peripheral nerve axon. J. Cell Biol. Jul 1986; 103(1): 33-39.

Izant JG, McIntosh JR. Microtubule-associated proteins: a monoclonal antibody to MAP2 binds to differentiated neurons. Proc. Natl. Acad. Sci. USA. Aug 1980; 77(8): 4741-4745.

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