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IP-10 Antibody, mAb, Mouse  |
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| Full Name |
IP-10 Antibody, mAb, Mouse
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Abbreviated name-1 |
Interferon-γ-Inducible Protein-10 human; CXCL10 |
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Description |
Mouse Anti-IP-10 Monoclonal Antibody reacts with recombinant human IP-10 fusion protein. The immunogen used to produce Mouse Anti IP-10 antibody was recombinant human IP-10 expressed in PET32a produced in E. coli. |
Ig Subclass |
Mouse IgG2b |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
Immunogen |
Purified recombinant human IP-10 expressed in PET32a |
Purification Method |
Protein G affinity column |
Reconstitution |
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 1.0-2.0 µg/ml |
Fusion Partner |
Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells. |
Conjugation |
Unconjugated |
Species Reactivity |
Human,Reactivity to other species is not tested yet. |
Host Species |
Mouse |
Antigen Species |
Human |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 4140_20080228014058.PDF (PDF)