c-Myc-tag Antibody [HRP], pAb, Rabbit
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c-Myc-tag Antibody [HRP], pAb, Rabbit  |
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![c-Myc-tag Antibody [HRP], pAb, Rabbit](/images/cgi2007/table2.gif "c-Myc-tag Antibody [HRP], pAb, Rabbit")
| Full Name |
c-Myc-tag Antibody [HRP], pAb, Rabbit
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Abbreviated name-1 |
Anti-c-Myc-tag [HRP] |
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Description |
Anti-c-Myc-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain c-Myc-tags and it is supplied as a 40 μg aliquot at a concentration of 1 mg/ml. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
This Antibody recognizes c-Myc tagged fusion proteins. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal. |
Immunogen |
c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized |
Conjugation |
Horseradish peroxidase (HRP) |
Host Species |
Rabbit |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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Related Products
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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![c-Myc-tag Antibody [HRP], pAb, Rabbit](/images/download_PDF.jpg "c-Myc-tag Antibody [HRP], pAb, Rabbit")
MSDS: 20080228011806 (PDF)![Rabbit Anti c-Myc-tag [HRP] (polyclonal) Western Blot](/site2/document/2481_20061220211122.JPG "Rabbit Anti c-Myc-tag [HRP] (polyclonal) Western Blot")
![c-Myc-tag Antibody [HRP], pAb, Rabbit](/images/zoom_in.jpg "c-Myc-tag Antibody [HRP], pAb, Rabbit")
