|
His-tag Antibody, pAb, Rabbit  |
|
|
| Full Name |
His-tag Antibody, pAb, Rabbit
|
Abbreviated name-1 |
His-tag Antibody, pAb, Rabbit; |
|
Description |
His-tag Antibody, pAb, Rabbit recognizes His tagged proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. |
Ig Subclass |
IgG |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags. |
Concentration |
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
His epitope tag peptide HHHHHH conjugated to KLH |
Purification Method |
Immunoaffinity chromatography |
Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml. |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized |
Conjugation |
Unconjugated |
Host Species |
Rabbit |
Note |
For N-terminal His-tagged protein detection, GenScript recommend THETM Anti-His mAb (Cat. No. A00186). |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
|
 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
|
Document-MSDS: 4117_20080228011555.PDF (PDF)
