Trx-tag Antibody, mAb, Mouse

This antibody reacts with the Thioredoxin fusion protein from E.coli and human.Not yet tested in other species.

Price	$102.00
Cat. No.	A00180-40
Size	40 μg Buy in bulk
Quantity
Est. Delivery


Ask us a question

Overview Applications Properties Background Bottom Notes

Overview

Specificity	This antibody reacts with the Thioredoxin fusion protein from E.coli and human.Not yet tested in other species.
Host Species	Mouse
Immunogen	Purified recombinant Trx-tag fusion protein
Species Reactivity	E.coli and human
Conjugate	Unconjugated

Back

Applications

Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.

Application	Recommended Usage
ELISA	0.1-1.0 µg/ml
Western Blot	1.0 µg/ml
Western Blot	1:100-1:1,000

Back

Properties

Form	Lyophilized
Storage Buffer	Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions	The lyophilized product remains stable for 1 year at -20℃ from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8℃ or for 12 months at -20℃ or below. Avoid repeated freezing and thawing cycles.
Purification	Protein A affinity column
Isotype	Mouse IgG2a
Clonality	Monoclonal
Clone ID	4C12H10

Back

Background

Target Background	Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen.Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.
Synonyms	Mouse Anti-Trx-tag;Anti-Trx-tag

Back

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Citations

1. Predrag Jevtić, et al. An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos. J Exp Biol. (2022-02)
2. Predrag Jevtić, et al. An insect antifreeze protein from Anatolica polita enhances the cryoprotection of Xenopus laevis eggs and embryos. J Exp Biol. (2022-02)
3. Wang Zhaoyu, et al. The RBG-1-RBG-2 complex modulates autophagy activity by regulating lysosomal biogenesis and function in C. elegans. J Cell Sci. (2019)
4. JancovichJames K, et al. Immunization of Pigs by DNA Prime and Recombinant Vaccinia Virus Boost To Identify and Rank African Swine Fever Virus Immunogenic and Protective Proteins. J. Virol. (2018-01)