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Cat. No.
 Name
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 Selected
A00180-40
40 μg
$79
A00180-200
200 μg(5x40μg)
$275
A00180-100
100 μg
$198
Trx-tag Antibody, mAb, Mousecustom antibody production
Trx-tag Antibody, mAb, Mouse
Full Name Trx-tag Antibody, mAb, Mouse
Abbreviated name-1
 Mouse Anti-Trx-tag; Anti-Trx-tag
Documents
Trx-tag Antibody, mAb, MouseDocument-MSDS: 4124_20080228012615.PDF (PDF)
Trx-tag Antibody, mAb, MouseDocument-MSDS: NaN3 MSDS.pdf (pdf)
Trx-tag Antibody, mAb, MouseDocument-COA: 13905_20110907015006.PDF (PDF)
Figures
Trx-tag Antibody, mAb, Mouse zoomTrx-tag Antibody, mAb, Mouse
Trx-tag Antibody, mAb, Mouse zoomTrx-tag Antibody, mAb, Mouse
Reference
Description
 Mouse Anti-Trx-tag Monoclonal Antibody reacts with the 109-aa Trx-tag sequence encoded by the PET-32a expression vectors from Novagen. Mouse Anti-Trx-tag Monoclonal Antibody is suitable for detecting fusion proteins that contain a Trx-tag.
Key Features
  • GenScript Trx-tag antibody has high sensitivity.
  • GenScript Trx-tag antibody can recognize N-terminal, C-terminal and internal Trx-tag in fusion proteins.
    		•
    Ig Subclass
    		 Mouse IgG2a
    Storage
    		 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
    Specificity
    		 This antibody reacts with the Thioredoxin fusion protein from E. coli and human. Not yet tested in other species.
    Concentration
    		 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
    Immunogen
    		 Purified recombinant Trx-tag fusion protein
    Purification Method
    		 Protein A affinity column
    Reconstitution
    		 Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
    Applications
    		 Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.1-1.0 µg/ml
    Western blot: 1.0 µg/ml
    Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 1.2 µg of this antibody is mixed with diluted WB solution (dilute 2 ml WB with 2ml of PBST).
    Other applications: user-optimized
    Fusion Partner
    		 Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
    Conjugation
    		 Unconjugated
    Species Reactivity
    		 E. coli and human
    Host Species
    		 Mouse
    Technical Manual
    		 ELISA Protocol (pdf)
    Western Blot Protocol (pdf)
    FACS Protocol (pdf)
    IHC Protocol (pdf)
    IP Protocol (pdf)
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    1
    		 Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
    2
    		 Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
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    		 Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.