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cGFP-tag Antibody, mAb, Mouse  |
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| Full Name |
cGFP-tag Antibody, mAb, Mouse
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Abbreviated name-1 |
Mouse Anti-cGFP-tag mAb; Green Fluorescent Protein |
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Description |
The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (GenScript, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses. |
Key Features |
GenScript cGFP-tag antibody has high sensitivity.
GenScript cGFP-tag antibody can recognize various forms of GFP proteins including eGFP, OFP and GFPuv. |
Ig Subclass |
Mouse IgG2b |
Storage |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv. |
Concentration |
1 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
Immunogen |
Purified recombinant cGFP fusion protein |
Purification Method |
Protein G affinity column |
Reconstitution |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1 mg/ml. |
Applications |
The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubations
ELISA: 0.1-1.0 µg/ml
Western blot: 1.0-2.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 4 µg of this antibody is mixed with 4 ml of WB solution.
Other applications: user-optimized |
Fusion Partner |
Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
Conjugation |
Unconjugated |
Host Species |
Mouse |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 4132_20080228013416.PDF (PDF)
