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A00191-40
40 μg
$90
A00191-100
100 μg
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GAPDH Antibody, pAb, Goatcustom antibody production
GAPDH Antibody, pAb, Goat
Full Name GAPDH Antibody, pAb, Goat
Abbreviated name-1
Anti-GAPDH;
Documents
GAPDH Antibody, pAb, GoatDocument-MSDS: 4143_20080228014557.PDF (PDF)
GAPDH Antibody, pAb, GoatDocument-MSDS: NaN3 MSDS.pdf (pdf)
GAPDH Antibody, pAb, GoatDocument-COA: 10K000209_A00191_COA.pdf (pdf)
Figures
GAPDH Antibody, pAb, Goat zoomGAPDH Antibody, pAb, Goat
GAPDH Antibody, pAb, Goat zoomGAPDH Antibody, pAb, Goat
GAPDH Antibody, pAb, Goat zoomGAPDH Antibody, pAb, Goat
Reference
Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often used as a control in experiments quantifying specific changes in expression levels of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes, such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimers disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.GenScript Goat Anti-GAPDH Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography.
Ig Subclass
Goat IgG
Storage
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
GenScript Goat Anti-GAPDH Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species.
Concentration
0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Immunogen
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle
Purification Method
Immunoaffinity chromatography
Reconstitution
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Applications
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Conjugation
Unconjugated
Species Reactivity
Human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish and E. coli
Host Species
Goat
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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1
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