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GAPDH Antibody [HRP], pAb, Goat

Cat. No.	Name	Size	Price
A00192-200	GAPDH Antibody [HRP], pAb, Goat	200 μg(5x40μg)	$270
A00192-40	GAPDH Antibody [HRP], pAb, Goat	40 μg	$60
A00192S	GAPDH Antibody [HRP], pAb, Goat	10 μg	Quote

Full Name

GAPDH Antibody [HRP], pAb, Goat

Abbreviated name-1

Anti-GAPDH [HRP];

Documents
Document-MSDS: 7123_20081021024451.PDF (PDF)
Document-MSDS: 9195_20090114012119.PDF (PDF)
Figures
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Reference

Description

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often measured as a control in experiments quantifying specific changes in expression of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimers disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Ig Subclass

Goat IgG

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

GenScript Goat Anti-GAPDH [HRP] Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species.

Concentration

0.5 mg/ml, lyophilized with PBS, pH7.4, containing 1%BSA, 0.01% thimerosal

Immunogen

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle

Purification Method

Immunoaffinity chromatography

Reconstitution

Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Conjugation

Horseradish peroxidase (HRP)

Species Reactivity

Human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

Related Products

Cat. No.	Name	Size	Price
A00084	GAPDH Antibody, mAb, Mouse	100 μg	$259
A00191-40	GAPDH Antibody, pAb, Goat	40 μg	$50
A00191-200	GAPDH Antibody, pAb, Goat	200 μg(5x40μg)	$225
A00915-40	GAPDH Antibody [Biotin], pAb, Goat	40 μg	$60
A00915-200	GAPDH Antibody [Biotin], pAb, Goat	200 μg(5x40μg)	$270


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