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NF-κB p65 Antibody (Phospho-Thr435), pAb, Rabbit



Cat. No.
 Name
Size   
Price
 Selected
A00221-100
 NF-κB p65 Antibody (Phospho-Thr435), pAb, Rabbit
100 μg
$188
NF-κB p65 Antibody (Phospho-Thr435), pAb, Rabbitcustom antibody production
NF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, Rabbit
Full Name NF-κB p65 Antibody (Phospho-Thr435), pAb, Rabbit
Abbreviated name-1
 Anti-NF-κB p65 (Phospho-Thr435);
Documents
NF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, RabbitDocument-MSDS: 7194_20081022025221.PDF (PDF)
NF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, RabbitDocument-MSDS: 3739_20080118034804.PDF (PDF)
Figures
NF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, Rabbit zoomNF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, Rabbit
NF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, Rabbit zoomNF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, Rabbit
NF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, Rabbit zoomNF-κB p65 Antibody (Phospho-Thr<sup>435</sup>), pAb, Rabbit
Reference
Description
 none
Ig Subclass
 Rabbit IgG
Storage
 Store at -20°C/1 year.
Specificity
 GenScript Rabbit Anti-NF-κB p65 (Phospho-Thr435) Polyclonal Antibody detects endogenous levels of NF-κB p65 only when phosphorylated at threonine 435.
Concentration
 1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150 mM NaCl, 0.02% sodium azide and 50% glycerol
Immunogen
 Synthesized phosphopeptide derived from human NF-κB around the phosphorylation site of threonine 435 (E-G-TP-L-S)
Purification Method
 The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Predicted Band Size
 65 kD
Observed Band Size
 65 kD
Applications
 WB: 1:500-1:1,000
IHC: 1:50-1:100
IF:1:100-1:200
Conjugation
 Unconjugated
Species Reactivity
 Human,mouse,rat
Host Species
 Rabbit
Antigen Species
 Human
Note
 Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
 TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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 NF-κB p65 Antibody (Ab-435), pAb, Rabbit
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