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Akt Antibody (Phospho-Ser⁴⁷³), pAb, Rabbit

Full Name

Abbreviated name-1

Rabbit Anti-Akt (Phospho-Ser⁴⁷³) pAb;

Documents
Document-MSDS: 7307_20081028013202.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
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Reference

Description

none

Ig Subclass

Rabbit IgG

Storage

Store at -20°C/1 year

Specificity

GenScript Rabbit Anti-Akt (Phospho-Ser⁴⁷³) Polyclonal Antibody detects endogenous levels of Akt only when phosphorylated at serine 473.

Concentration

1 mg/ml in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol

Immunogen

Synthesized phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-S^P-Y-S)

Purification Method

GenScript Rabbit Anti-Akt (Phospho-Ser⁴⁷³) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Predicted Band Size

60 kD

Observed Band Size

60 kD

Applications

WB: 1:500-1:1,000
IHC: 1:50-1:100

Conjugation

Unconjugated

Species Reactivity

Human, mouse, rat

Host Species

Rabbit

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

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A00965-40	Akt Antibody (Phospho-Ser⁴⁷³), pAb, Rabbit	40 μg	$69
A00965-200	Akt Antibody (Phospho-Ser⁴⁷³), pAb, Rabbit	200 μg(5x40μg)	$311
RP19637	Akt (Phospho-Ser⁴⁷³) pAb Blocking Peptide	100 μg	$50


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2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3	Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.

Akt Antibody (Phospho-Ser473), pAb, Rabbit

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Akt Antibody (Phospho-Ser⁴⁷³), pAb, Rabbit