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PTEN Antibody (Phospho-Ser³⁸⁰, Thr^{382, 383}), pAb, Rabbit

Full Name

Abbreviated name-1

Anti PTEN (Phospho-Ser³⁸⁰, Thr^{382, 383});

Documents
Document-MSDS: 7317_20081028015241.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

none

Ig Subclass

Rabbit IgG

Storage

Store at -20°C/1 year.

Specificity

GenScript Rabbit Anti-PTEN (Phospho-Ser³⁸⁰, Thr^{382, 383}) Polyclonal Antibody detects endogenous levels of PTEN only when phosphorylated at serine 380 and threonine 382, 383.

Concentration

1 mg/ml in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol

Immunogen

Synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 380 and threonine 382, 383 (R-Y-S^P-D-T^P-T^P-D-S)

Purification Method

GenScript Rabbit Anti-PTEN (Phospho-Ser³⁸⁰, Thr^{382, 383}) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Predicted Band Size

54 kD

Observed Band Size

54 kD

Applications

WB: 1:500-1:1,000
IHC: 1:50-100
IF:1:100-1:200

Conjugation

Unconjugated

Species Reactivity

Human,mouse,rat

Host Species

Rabbit

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)


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2	Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
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PTEN Antibody (Phospho-Ser380, Thr382, 383), pAb, Rabbit

PTEN Antibody (Phospho-Ser³⁸⁰, Thr^{382, 383}), pAb, Rabbit