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PTEN Antibody (Phospho-Ser<sup>380</sup>, Thr<sup>382, 383</sup>), pAb, Rabbit
Full Name
PTEN Antibody (Phospho-Ser380, Thr382, 383), pAb, Rabbit PTEN Antibody (Phospho-Ser<sup>380</sup>, Thr<sup>382, 383</sup>), pAb, Rabbit
Abbreviated name-1
Anti PTEN (Phospho-Ser380, Thr382, 383);
Documents
Description
none
Ig Subclass
Rabbit IgG
Storage
Store at -20°C/1 year.
Specificity
GenScript Rabbit Anti-PTEN (Phospho-Ser380, Thr382, 383) Polyclonal Antibody detects endogenous levels of PTEN only when phosphorylated at serine 380 and threonine 382, 383.
Formulation
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
Immunogen
Synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 380 and threonine 382, 383 (R-Y-SP-D-TP-TP-D-S)
Purification Method
GenScript Rabbit Anti-PTEN (Phospho-Ser380, Thr382, 383) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Predicted Band Size
54 kD
Observed Band Size
54 kD
Applications
WB: 1:500-1:1,000
IHC: 1:50-100
IF:1:100-1:200
Conjugation
Unconjugated
Species Reactivity
Human,mouse,rat
Host Species
Rabbit
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
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