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Akt Antibody (Ab-473), pAb, Rabbit

Full Name

Abbreviated name-1

Rabbit Anti-Akt (Ab-473) pAb; Anti Akt

Documents
Document-MSDS: 7392_20081028224450.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
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Reference

Description

none

Ig Subclass

Rabbit IgG

Storage

Store at -20°C/1 year.

Specificity

GenScript Rabbit Anti-Akt (Ab-473) Polyclonal Antibody detects endogenous levels of total Akt protein.

Concentration

1 mg/ml in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol

Immunogen

Synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-S^P-Y-S)

Purification Method

The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Predicted Band Size

60 kD

Observed Band Size

60 kD

Applications

WB: 1:100-1:500
IHC: 1:50-1:100

Conjugation

Unconjugated

Species Reactivity

Human, mouse, rat

Host Species

Rabbit

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

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