antibody » antibody : HER2 Antibody (Phospho-Tyr877), pAb, Rabbit
Cat. No.
Name
Size   
Price
Selected
A00313-100
100 μg
$188
HER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, Rabbit
Full Name HER2 Antibody (Phospho-Tyr877), pAb, Rabbit
Abbreviated name-1
Rabbit Anti HER2 (Phospho-Tyr877);
Documents
HER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, RabbitDocument-MSDS: 7418_20081028233827.PDF (PDF)
HER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, RabbitDocument-MSDS: NaN3 MSDS.pdf (pdf)
Figures
HER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, Rabbit zoomHER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, Rabbit
HER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, Rabbit zoomHER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, Rabbit
HER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, Rabbit zoomHER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, Rabbit
Description
none
Ig Subclass
Rabbit IgG
Storage
Store at -20°C/1 year.
Specificity
GenScript Rabbit Anti-HER2 (Phospho-Tyr877) Polyclonal Antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 877.
Formulation
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
Immunogen
Synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 877 (T-E-YP-H-A)
Purification Method
GenScript Rabbit Anti-HER2 (Phospho-Tyr877) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.
Predicted Band Size
185 kD
Observed Band Size
185 kD
Applications
WB: 1:500-1:1,000
IHC: 1:50-1:100
IF:1:100-1:200
Conjugation
Unconjugated
Species Reactivity
Human,mouse,rat
Host Species
Rabbit
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
Document-COA
1
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool.
2
Polyclonal antibody Services: GenScript's custom polyclonal antibody services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced antigen design tool.
3
Custom Monoclonal Antibody Services: GenScript's custom monoclonal antibody service guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced professional.