HER2 Antibody (Phospho-Tyr877), pAb, Rabbit- GenScript, AAALAC International Accredited Animal Facility

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HER2 Antibody (Phospho-Tyr⁸⁷⁷), pAb, Rabbit

Full Name

HER2 Antibody (Phospho-Tyr⁸⁷⁷), pAb, Rabbit

Abbreviated name-1

Rabbit Anti HER2 (Phospho-Tyr⁸⁷⁷);

Documents

HER2 Antibody (Phospho-Tyr<sup>877</sup>), pAb, Rabbit

Document-MSDS: 7418_20081028233827.PDF (PDF)

Document-MSDS: NaN3 MSDS.pdf (pdf)

Document-COA: C59841209_A00313_COA.pdf (PDF)

Figures

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Reference

Description

none

Ig Subclass

Rabbit IgG

Storage

Store at -20°C/1 year.

Specificity

GenScript Rabbit Anti-HER2 (Phospho-Tyr⁸⁷⁷) Polyclonal Antibody detects endogenous levels of HER2 only when phosphorylated at tyrosine 877.

Concentration

1 mg/ml in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol

Immunogen

Synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 877 (T-E-Y^P-H-A)

Purification Method

GenScript Rabbit Anti-HER2 (Phospho-Tyr⁸⁷⁷) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorrylation site.

Predicted Band Size

185 kD

Observed Band Size

185 kD

Applications

WB: 1:500-1:1,000
IHC: 1:50-1:100
IF:1:100-1:200

Conjugation

Unconjugated

Species Reactivity

Human,mouse,rat

Host Species

Rabbit

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)


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