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p27Kip1 Antibody (Phospho-Thr187), pAb, Rabbit



Cat. No.
 Name
Size   
Price
 Selected
A00333-100
 p27Kip1 Antibody (Phospho-Thr187), pAb, Rabbit
100 μg
$188
p27Kip1 Antibody (Phospho-Thr187), pAb, Rabbitcustom antibody production
p27Kip1 Antibody (Phospho-Thr<sup>187</sup>), pAb, Rabbit
Full Name p27Kip1 Antibody (Phospho-Thr187), pAb, Rabbit
Abbreviated name-1
 Anti p27Kip1 (Phospho-Thr187);
Documents
p27Kip1 Antibody (Phospho-Thr<sup>187</sup>), pAb, RabbitDocument-MSDS: 7468_20081029031217.PDF (PDF)
p27Kip1 Antibody (Phospho-Thr<sup>187</sup>), pAb, RabbitDocument-MSDS: 3739_20080118034804.PDF (PDF)
Figures
p27Kip1 Antibody (Phospho-Thr<sup>187</sup>), pAb, Rabbit zoomp27Kip1 Antibody (Phospho-Thr<sup>187</sup>), pAb, Rabbit
p27Kip1 Antibody (Phospho-Thr<sup>187</sup>), pAb, Rabbit zoomp27Kip1 Antibody (Phospho-Thr<sup>187</sup>), pAb, Rabbit
Reference
Description
 none
Ig Subclass
 Rabbit IgG
Storage
 Store at -20°C/1 year.
Specificity
 Rabbit Anti-p27Kip1 (Phospho-Thr187) detects endogenous levels of p27Kip1 only when phosphorylated at threonine 187.
Concentration
 1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
Immunogen
 Synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of threonine 187(E-Q-TP-P-K)
Purification Method
 The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Predicted Band Size
 27 kD
Observed Band Size
 27 kD
Applications
 Western blot: 1:500-1:1,000
IHC:1:50-1:100
Conjugation
 Unconjugated
Species Reactivity
 Human, mouse, rat
Host Species
 Rabbit
Antigen Species
 Human
Note
 Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
 TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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