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HDAC5 Antibody (Phospho-Ser<sup>498</sup>), pAb, Rabbit
Full Name
HDAC5 Antibody (Phospho-Ser498), pAb, Rabbit HDAC5 Antibody (Phospho-Ser<sup>498</sup>), pAb, Rabbit
Abbreviated name-1
Anti HDAC5 (Phospho-Ser498);
Documents
Description
GenScript Rabbit Anti-HDAC5 (Phospho-Ser498) Polyclonal Antibody is highly purified from Rabbit antiserum by The antibody is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. The antibody is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, containing 0.02% sodium azide.
Ig Subclass
Rabbit IgG
Storage
GenScript Rabbit Anti-HDAC5 (Phospho-Ser498) Polyclonal Antibody will remain stable for up to one year if stored at -20°C.
Specificity
GenScript Rabbit Anti-HDAC5 (Phospho-Ser498) Polyclonal Antibody detects endogenous levels of HDAC5 only when phosphorylated at serine 498.
Formulation
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
Immunogen
Synthesized phosphopeptide derived from human HDAC5 around the phosphorylation site of Serine498 (T-Q-SP-S-P).
Purification Method
GenScript Rabbit Anti-HDAC5 (Phospho-Ser498) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Predicted Band Size
124 kD
Observed Band Size
124 kD
Applications
Western blot: 1:500-1:1,000
IHC: 1:50-1:100
Conjugation
Unconjugated
Species Reactivity
Human, mouse, rat
Host Species
Rabbit
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Citation
Bhatnagar A.,et al. Interaction of G-Protein βγ Complex with Chromatin Modulates GPCR-Dependent Gene Regulation.PLoS One.2013Jan;8(1):e52689

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