| Full Name |
HDAC5 Antibody (Phospho-Ser498), pAb, Rabbit
|
Abbreviated name-1 |
Anti HDAC5 (Phospho-Ser498); |
|
Description |
GenScript Rabbit Anti-HDAC5 (Phospho-Ser498) Polyclonal Antibody is highly purified from Rabbit antiserum by The antibody is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. The antibody is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, containing 0.02% sodium azide. |
Ig Subclass |
Rabbit IgG |
Storage |
GenScript Rabbit Anti-HDAC5 (Phospho-Ser498) Polyclonal Antibody will remain stable for up to one year if stored at -20°C. |
Specificity |
GenScript Rabbit Anti-HDAC5 (Phospho-Ser498) Polyclonal Antibody detects endogenous levels of HDAC5 only when phosphorylated at serine 498. |
Concentration |
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol |
Immunogen |
Synthesized phosphopeptide derived from human HDAC5 around the phosphorylation site of Serine498 (T-Q-SP-S-P). |
Purification Method |
GenScript Rabbit Anti-HDAC5 (Phospho-Ser498) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
Predicted Band Size |
124 kD |
Observed Band Size |
124 kD |
Applications |
Western blot: 1:500-1:1,000
IHC: 1:50-1:100 |
Conjugation |
Unconjugated |
Species Reactivity |
Human, mouse, rat |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
|
Document-MSDS: 7478_20081029032204.PDF (PDF)
