AMPKα1 Antibody (Phospho-Ser487), pAb, Rabbit
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AMPKα1 Antibody (Phospho-Ser 487), pAb, Rabbit  |
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| Full Name |
AMPKα1 Antibody (Phospho-Ser487), pAb, Rabbit
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Abbreviated name-1 |
AMPKα1 Antibody (Phospho-Ser487), pAb, Rabbit; |
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Description |
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-COA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-COA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. |
Ig Subclass |
Rabbit IgG |
Storage |
Store at -20°C/1 year. |
Specificity |
GenScript Rabbit Anti-AMPKα1 (Phospho-Ser487) Polyclonal Antibody detects endogenous levels of AMPKα1 only when phosphorylated at serine 487. |
Concentration |
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol |
Immunogen |
Synthesized phosphopeptide derived from human AMPKα1 around the phosphorylation site of Serine 487 (S-G-SP-V-S) |
Purification Method |
GenScript Rabbit Anti-AMPKα1 (Phospho-Ser487) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
Predicted Band Size |
63 kD |
Observed Band Size |
63 kD |
Applications |
Western blot: 1:500-1:1,000
IHC: 1:50-1:100
IF: 1:100-1:200 |
Conjugation |
Unconjugated |
Species Reactivity |
Human, Mouse, Rat |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)
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Related Products
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1 |
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Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
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Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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Document-MSDS: 7498_20081029035808.PDF (PDF)
