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MEK1 Antibody (Phospho-Ser²²¹), pAb, Rabbit

Full Name

Abbreviated name-1

Anti MEK1 (Phospho-Ser²²¹);

Documents
Document-MSDS: 7637_20081102195819.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
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Reference

Description

none

Ig Subclass

Rabbit IgG

Storage

Store at -20°C/1 year.

Specificity

GenScript Rabbit Anti-MEK1 (Phospho-Ser²²¹) Polyclonal Antibody detects endogenous levels of MEK1 only when phosphorylated at serine 221.

Concentration

1 mg/ml in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol

Immunogen

Synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of serine 221 (A-N-S^P-F-V)

Purification Method

The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Predicted Band Size

43 kD

Observed Band Size

43 kD

Applications

Western blot: 1:500-1:1,000
IHC: 1:50-1:100
IF: 1:100-1:200

Conjugation

Unconjugated

Species Reactivity

Human, mouse, rat

Host Species

Rabbit

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

Related Products

Cat. No.	Name	Size	Price
A00452-100	MEK1 Antibody (Ab-221), pAb, Rabbit	100 μg	$140
RP19813	MEK1 (Phospho-Ser²²¹) pAb Blocking Peptide	100 μg	$50


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MEK1 Antibody (Phospho-Ser221), pAb, Rabbit

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MEK1 Antibody (Phospho-Ser²²¹), pAb, Rabbit