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p53 Antibody (Phospho-Ser 46), pAb, Rabbit  |
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| Full Name |
p53 Antibody (Phospho-Ser46), pAb, Rabbit
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Abbreviated name-1 |
Anti p53 (Phospho-Ser46); Anti p53 |
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Description |
none |
Ig Subclass |
Rabbit IgG |
Storage |
Store at -20°C/1 year. |
Specificity |
GenScript Rabbit Anti-p53 (Phospho-Ser46) Polyclonal Antibody detects endogenous levels of p53 only when phosphorylated at serine 46. |
Concentration |
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol |
Immunogen |
Synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 46 (M-L-SP-P-D). |
Purification Method |
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
Predicted Band Size |
53 kD |
Observed Band Size |
53 kD |
Applications |
WB: 1:500-1:1,000 IF: 1:100-1:200 |
Conjugation |
Unconjugated |
Species Reactivity |
Human |
Host Species |
Rabbit |
Antigen Species |
Human |
Note |
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. |
Technical Manual |
ELISA Protocol (pdf) Western Blot Protocol (pdf) FACS Protocol (pdf) IHC Protocol (pdf) IP Protocol (pdf)
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 |
1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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