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p53 Antibody (Phospho-Ser<sup>46</sup>), pAb, Rabbit
Full Name
p53 Antibody (Phospho-Ser46), pAb, Rabbit p53 Antibody (Phospho-Ser<sup>46</sup>), pAb, Rabbit
Abbreviated name-1
Anti p53 (Phospho-Ser46); Anti p53
Documents
Description
none
Ig Subclass
Rabbit IgG
Storage
Store at -20°C/1 year.
Specificity
GenScript Rabbit Anti-p53 (Phospho-Ser46) Polyclonal Antibody detects endogenous levels of p53 only when phosphorylated at serine 46.
Formulation
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
Immunogen
Synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 46 (M-L-SP-P-D).
Purification Method
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Predicted Band Size
53 kD
Observed Band Size
53 kD
Applications
WB: 1:500-1:1,000
IF: 1:100-1:200
Conjugation
Unconjugated
Species Reactivity
Human
Host Species
Rabbit
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
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