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BRCA1 Antibody (Phospho-Ser<sup>1524</sup>), pAb, Rabbit
Full Name
BRCA1 Antibody (Phospho-Ser1524), pAb, Rabbit BRCA1 Antibody (Phospho-Ser<sup>1524</sup>), pAb, Rabbit
Abbreviated name-1
Rabbit Anti-BRCA1 (Phospho-Ser1524) pAb;
Documents
Description
none
Ig Subclass
Rabbit IgG
Storage
Store at -20°C/1 year
Specificity
Rabbit Anti BRCA1 (phospho-Ser1524) detects endogenous levels of BRCA1 only when phosphorylated at serine 1524.
Formulation
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
Immunogen
Synthesized phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1524 (Y-P-SP-Q-E)
Purification Method
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Applications
IHC: 1:50-1:100
Conjugation
Unconjugated
Species Reactivity
Human
Host Species
Rabbit
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
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