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Histone H2A.X Antibody (Ab-139), pAb, Rabbit

Full Name

Abbreviated name-1

Anti Histone H2A.X (Ab-139); Anti Histone H2A.X

Documents
Document-MSDS: 7563_20081030020122.PDF (PDF)
Document-MSDS: 3739_20080118034804.PDF (PDF)
Figures
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Reference

Description

none

Ig Subclass

Rabbit IgG

Storage

Store at -20°C/1 year.

Specificity

GenScript Rabbit Anti-Histone H2A.X (Ab-139) Polyclonal Antibody detects endogenous levels of total Histone H2A.X protein.

Concentration

1 mg/ml in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol

Immunogen

Synthesized non-phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-S^P-Q-E)

Purification Method

GenScript Rabbit Anti-Histone H2A.X (Ab-139) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Predicted Band Size

15 kD

Observed Band Size

15 kD

Applications

Western blot: 1:500-1:1,000
IHC: 1:50-1:100
IF: 1:100-1:200

Conjugation

Unconjugated

Species Reactivity

Human

Host Species

Rabbit

Antigen Species

Human

Note

Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

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