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Connexin 43 Antibody (Phospho-Ser<sup>367</sup>), pAb, Rabbit
Full Name
Connexin 43 Antibody (Phospho-Ser367), pAb, Rabbit Connexin 43 Antibody (Phospho-Ser<sup>367</sup>), pAb, Rabbit
Abbreviated name-1
Anti Connexin 43 (Phospho-Ser367); Anti Connexin 43
Documents
Description
none
Ig Subclass
Rabbit IgG
Storage
Store at -20°C/1 year.
Specificity
Rabbit Anti-Connexin 43 (Phospho-Ser367) Polyclonal Antibody detects endogenous levels of Connexin 43 only when phosphorylated at serine 367.
Formulation
1 mg/ml in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol
Immunogen
Synthesized phosphopeptide derived from human Connexin 43 around the phosphorylation site of serine 367 (R-A-SP-S-R).
Purification Method
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Predicted Band Size
43 kD
Observed Band Size
43 kD
Applications
Western blot: 1:500-1:1,000
IHC: 1:50-1:100
Conjugation
Unconjugated
Species Reactivity
Human, mouse, rat
Host Species
Rabbit
Antigen Species
Human
Note
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Technical Manual
ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
Related Products
Cat. No.
Product Name
Size
Price
Quantity
A00544-100 Connexin 43 Antibody (Ab-367), pAb, Rabbit
100 μg
$140.00
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