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Cat. No.
 Name
Size   
Price
 Selected
A00612
100 μg
$410
THETM His Tag Antibody [HRP], mAb, Mousecustom antibody production
THE<sup>TM</sup> His Tag Antibody [HRP], mAb, Mouse
Full Name THETM His Tag Antibody [HRP], mAb, Mouse
Abbreviated name-1
 His-tag Antibody [HRP],mAb, Mouse;
Documents
THE<sup>TM</sup> His Tag Antibody [HRP], mAb, MouseDocument-MSDS: 3891_20080213022807.PDF (PDF)
THE<sup>TM</sup> His Tag Antibody [HRP], mAb, MouseDocument-MSDS: 9195_20090114012119.PDF (PDF)
THE<sup>TM</sup> His Tag Antibody [HRP], mAb, MouseDocument-COA: 11K000403_A00612_COA.pdf (pdf)
THE<sup>TM</sup> His Tag Antibody [HRP], mAb, MouseDocument-COA: 11I000355_A00612_COA.pdf (pdf)
THE<sup>TM</sup> His Tag Antibody [HRP], mAb, MouseDocument-COA: 11G000323_A00612_COA.pdf (pdf)
THE<sup>TM</sup> His Tag Antibody [HRP], mAb, MouseDocument-COA: 11A000257_A00612_COA.pdf (pdf)
Figures
THE<sup>TM</sup> His Tag Antibody [HRP], mAb, Mouse zoomTHE<sup>TM</sup> His Tag Antibody [HRP], mAb, Mouse
Reference
Description
 GenScript THETM His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-His Monoclonal Antibody (Mouse) (A00186) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.
Ig Subclass
 IgG1, k
Storage
 The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Specificity
 THETM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Concentration
 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Immunogen
 A synthetic peptide HHHHHH coupled to KLH
Purification Method
 Protein A affinity column
Reconstitution
 Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.
Applications
 Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Conjugation
 Horseradish Peroxidase (HRP)
Host Species
 Mouse
Technical Manual
 ELISA Protocol (pdf)
Western Blot Protocol (pdf)
FACS Protocol (pdf)
IHC Protocol (pdf)
IP Protocol (pdf)
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