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THE^TM His Tag Antibody [HRP], mAb, Mouse

Full Name

Abbreviated name-1

His-tag Antibody [HRP],mAb, Mouse;

Documents
Document-MSDS: 3891_20080213022807.PDF (PDF)
Document-MSDS: 9195_20090114012119.PDF (PDF)
Document-COA: 14279_20111114035432.PDF (PDF)
Document-COA: 14280_20111114035432.PDF (PDF)
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Description

GenScript THE^TM His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THE^TM Anti-His Monoclonal Antibody (Mouse) (A00186) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.

Ig Subclass

IgG1, k

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Specificity

THE^TM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal

Immunogen

A synthetic peptide HHHHHH coupled to KLH

Purification Method

Protein A affinity column

Reconstitution

Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Conjugation

Horseradish Peroxidase (HRP)

Host Species

Mouse

Technical Manual

TM0589.pdf (PDF)
TM0590.pdf (PDF)
TM0591.pdf (PDF)
TM0592.pdf (PDF)
TM0593.pdf (PDF)

Related Products

Cat. No.	Name	Size	Price
A00174-40	His-tag Antibody, pAb, Rabbit	40 μg	$50
A00174-200	His-tag Antibody, pAb, Rabbit	200 μg(5x40μg)	$225
A00186-100	THE^TM His Tag Antibody, mAb, Mouse	100 μg	$375
A00613	THE^TM His Tag Antibody [Biotin], mAb, Mouse	100 μg	$410


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THETM His Tag Antibody [HRP], mAb, Mouse

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THE^TM His Tag Antibody [HRP], mAb, Mouse