cGMP Antibody, pAb, Rabbit
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cGMP Antibody, pAb, Rabbit  |
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| Full Name |
cGMP Antibody, pAb, Rabbit
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Abbreviated name-1 |
Rabbit Anti-cGMP pAb |
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Description |
Guanosine 3', 5'-cyclic monophosphate (cyclic GMP; cGMP) acts as a secondary messenger much like cyclic AMP. It is generally known to activate intracellular protein kinases in response to the binding of membrane-impermeable peptide hormones to the cell surface. cGMP synthesis is catalyzed by guanylate cyclase (GC), which converts GTP to cGMP. Membrane-bound GC is activated by peptide hormones such as atrial natriuretic factor, while soluble GC is typically activated by nitric oxide (NO) to stimulate cGMP synthesis.
cGMP is also a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. It also relaxes smooth muscle tissues. The roles of cGMP and cAMP may be linked, as evidenced by the fact that some cellular functions are controlled bi-directionally by both cAMP and cGMP. Some functions are stimulated by cGMP and suppressed by cAMP and vice versa.
GenScript Rabbit Anti-cGMP Polyclonal Antibody is developed in rabbit hosts using 3', 5'-cyclic GMP-8-KLH as immunogen. |
Ig Subclass |
Rabbit IgG |
Storage |
The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
Specificity |
The specificity of the antiserum is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. The cross-reactivity data obtained in competitive ELISA system is as follows:
| Compound | % Cross-reactivity |
|---|
| cGMP | 100 |
| AMP | <0.0001 |
| cAMP | <0.0001 |
| GMP | <0.0001 |
| ADP | <0.0001 |
| GDP | <0.0001 |
| ATP | <0.0001 |
| GTP | <0.0001 |
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Immunogen |
3', 5'-cyclic GMP-8-KLH |
Purification Method |
Antiserum |
Applications |
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may depend on secondary antibody affinity, antigen concentration, detection method sensitivity, temperature, incubations length, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are starting recommendation for this product.
ELISA: 1:4,000-1:40,000 |
Conjugation |
Unconjugated |
Antigen Synonyms |
cyclic GMP, 3',5'-cyclic GMP, 3',5'-cyclic guanosine monophosphate, guanosine 3',5'-cyclic monophosphate |
Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap. |
Technical Manual |
ELISA Protocol (PDF)
Western Blotting Protocol (PDF)
FACS Protocol (PDF)
IHC Protocol (PDF)
IP Protocol (PDF)
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Related Products
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1 |
Antibody Services: GenScript's custom antibody service guarantees 1:32,000 ELISA titer of final product or two ELISA positive clones with the help of our advanced antigen design tool. |
2 |
Polyclonal
antibody Services: GenScript's custom polyclonal antibody
services start from $250. It comes with a 1:32,000 ELISA titer guarantee, thanks to our advanced
antigen design tool. |
3 |
Custom
Monoclonal Antibody Services: GenScript's custom monoclonal antibody service
guarantees two ELISA positive clones with the help of advanced hybridoma technology & experienced
professional. |
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MSDS: 20080917232344 (PDF)
